ISOLATION OF CYTOTOXIC NEOLIGNANS FROM Saururus cernuus L. (SAURURACEAE) USING IONIC LIQUID IN THE MICROWAVE ASSISTED EXTRACTION (MAE)

Author(s)

Brito, Juliana R. ; Camilo, Fernanda F. ; Figueiredo, Carlos R. ; Azevedo, Ricardo A. ; Romoff, Paulete ; Buturi, Fatima O. S. ; Favero, Oriana A. ; Lago, Joao Henrique G. ; Ferreira, Edgard A.

• Document type: Journal article
• Source: Química Nova; v. 41, n. 7, p. 4-pg., 2018-07-01.

Abstract

In the present work, dried leaves of Sasrurus cernuus (Saururaceae) were subjected action using an ionic liquid (1-butyl-3-methylimidazolium bromide BMImBr) in the microwave assisted extraction (MAE). The obtained extract was partitioned using n-hexane and cytotoxicity activity of this organic phase against murine melanoma cell line (B16F10-Nex2) was evaluated in vitro. Since this extract displayed activity (100% of cell death at 200 mu g mL(-1)) it was subjected to a bioactivity-guided fractionation to afford four related neolignans: three-austrobailignan-5 threo-austrobailignan-6 (2), threo-dihydroguaiaretic acid (3) and saucernetin (4). Their chemical structures were established based on NMK and MS spectral analysis. Among the isolated neolignans, compound 2 exhibited the highest cytotoxic activity against HeLa (human cervical melanoma) cells with IC50 of 28.3 +/- 3.9 mu g mL(-1) (86 +/- 12 mu mol L-1). Furthermore, compounds 2 and 3 exhibited the highest cytotoxic activity against A2058 (human melanoma) cells with IC50 of 44.3 +/- 4.2 mu g (135 +/- 13 mol L-1) and 41.5 +/- 7.5 mu g (126 +/- 23 mu mol respectively, similar to positive control cisplatin (IC50 = 43.2 +/- 3.2 mu g or 144 +/- 11 mu mol L-1). Otherwise, compound 4 was inactive (IC50 > 100 mu g mL(-1) or > 300 mu mol L-1). The obtained results provide important data for the selection of bioactive neolignans with promising cytotoxic potential using a simple and fast method employing a green solvent as 1-butyl-3-methylimidazolium bromide (BMImBr). (AU)

• FAPESP's process: 15/11936-2 - Use of chemodiversity of plant species in remaining areas of Atlantic Forest from São Paulo State in the selection of biologically active prototypes
• Grantee: João Henrique Ghilardi Lago
• Support Opportunities: Regular Research Grants