Chromosomal Evolution and Evolutionary Relationships of Lebiasina Species (Characiformes, Lebiasinidae)

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Cavalcante Sassi, Francisco de Menezes ; de Oliveira, Ezequiel Aguiar ; Carlos Bertollo, Luiz Antonio ; Nirchio, Mauro ; Hatanaka, Terumi ; Ferreira Marinho, Manoela Maria ; Moreira-Filho, Orlando ; Aroutiounian, Rouben ; Liehr, Thomas ; Al-Rikabi, Ahmed B. H. ; Cioffi, Marcelo de Bello

• Document type: Journal article
• Source: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 20, n. 12, p. 17-pg., 2019-06-02.

Abstract

We present the first cytogenetic data for Lebiasina bimaculata and L. melanoguttata with the aim of (1) investigating evolutionary events within Lebiasina and their relationships with other Lebiasinidae genera and (2) checking the evolutionary relationships between Lebiasinidae and Ctenoluciidae. Both species have a diploid number 2n = 36 with similar karyotypes and microsatellite distribution patterns but present contrasting C-positive heterochromatin and CMA(3)(+) banding patterns. The remarkable interstitial series of C-positive heterochromatin occurring in L. melanoguttata is absent in L. bimaculata. Accordingly, L. bimaculata shows the ribosomal DNA sites as the only GC-rich (CMA(3)(+)) regions, while L. melanoguttata shows evidence of a clear intercalated CMA(3)(+) banding pattern. In addition, the multiple 5S and 18S rDNA sites in L. melanogutatta contrast with single sites present in L. bimaculata. Comparative genomic hybridization (CGH) experiments also revealed a high level of genomic differentiation between both species. A polymorphic state of a conspicuous C-positive, CMA(3)(+), and (CGG)n band was found only to occur in L. bimaculata females, and its possible relationship with a nascent sex chromosome system is discussed. Whole chromosome painting (WCP) and CGH experiments indicate that the Lebiasina species examined and Boulengerella maculata share similar chromosomal sequences, thus supporting the relatedness between them and the evolutionary relationships between the Lebiasinidae and Ctenoluciidae families. (AU)

• FAPESP's process: 18/24235-0 - Karyotype and sex chromosome evolution in Neotropical fishes. Part III.
• Grantee: Luiz Antonio Carlos Bertollo
• Support Opportunities: Regular Research Grants
• FAPESP's process: 18/22033-1 - An intercontinental approach for the investigation of chromosome evolution, genetic diversity and biogeography in the order Osteoglossiformes (Teleostei: Osteoglossomorpha). Part III
• Grantee: Marcelo de Bello Cioffi
• Support Opportunities: Regular Research Grants
• FAPESP's process: 17/09321-5 - Miniature Characiformes (Teleostei: Ostariophysi): evolution, diversity and biogeography
• Grantee: Manoela Maria Marinho Koh
• Support Opportunities: BIOTA-FAPESP Program - Young Investigators Grants