| Full text | |
| Author(s): Show less - |
Chatterjee, Sayantani
;
Lee, Ling Y.
;
Kawahara, Rebeca
;
Abrahams, Jodie L.
;
Adamczyk, Barbara
;
Anugraham, Merrina
;
Ashwood, Christopher
;
Sumer-Bayraktar, Zeynep
;
Briggs, Matthew T.
;
Chik, Jenny H. L.
;
Everest-Dass, Arun
;
Foerster, Sarah
;
Hinneburg, Hannes
;
Leite, Katia R. M.
;
Loke, Ian
;
Moginger, Uwe
;
Moh, Edward S. X.
;
Nakano, Miyako
;
Recuero, Saulo
;
Sethi, Manveen K.
;
Srougi, Miguel
;
Stavenhagen, Kathrin
;
Venkatakrishnan, Vignesh
;
Wongtrakul-Kish, Katherine
;
Diestel, Simone
;
Hoffmann, Peter
;
Karlsson, Niclas G.
;
Kolarich, Daniel
;
Molloy, Mark P.
;
Muders, Michael H.
;
Oehler, Martin K.
;
Packer, Nicolle H.
;
Palmisano, Giuseppe
;
Thaysen-Andersen, Morten
Total Authors: 34
|
| Document type: | Journal article |
| Source: | PROTEOMICS; v. 19, n. 21-22, p. 13-pg., 2019-10-16. |
| Abstract | |
While aberrant protein glycosylation is a recognized characteristic of human cancers, advances in glycoanalytics continue to discover new associations between glycoproteins and tumorigenesis. This glycomics-centric study investigates a possible link between protein paucimannosylation, an under-studied class of human N-glycosylation [Man(1-3)GlcNAc(2)Fuc(0-1)], and cancer. The paucimannosidic glycans (PMGs) of 34 cancer cell lines and 133 tissue samples spanning 11 cancer types and matching non-cancerous specimens are profiled from 467 published and unpublished PGC-LC-MS/MS N-glycome datasets collected over a decade. PMGs, particularly Man(2-3)GlcNAc(2)Fuc(1), are prominent features of 29 cancer cell lines, but the PMG level varies dramatically across and within the cancer types (1.0-50.2%). Analyses of paired (tumor/non-tumor) and stage-stratified tissues demonstrate that PMGs are significantly enriched in tumor tissues from several cancer types including liver cancer (p = 0.0033) and colorectal cancer (p = 0.0017) and is elevated as a result of prostate cancer and chronic lymphocytic leukaemia progression (p < 0.05). Surface expression of paucimannosidic epitopes is demonstrated on human glioblastoma cells using immunofluorescence while biosynthetic involvement of N-acetyl-beta-hexosaminidase is indicated by quantitative proteomics. This intriguing association between protein paucimannosylation and human cancers warrants further exploration to detail the biosynthesis, cellular location(s), protein carriers, and functions of paucimannosylation in tumorigenesis and metastasis. (AU) | |
| FAPESP's process: | 18/15549-1 - Post-translational modifications in Chagas Disease biological processes and diagnostics: novel methodological approaches and biological applications |
| Grantee: | Giuseppe Palmisano |
| Support Opportunities: | Research Grants - Young Investigators Grants - Phase 2 |
| FAPESP's process: | 17/03010-8 - Site-specific characterization of N- and O-linked glycosylation in prostate cancer tissues as molecular signature for disease progression |
| Grantee: | Rebeca Kawahara Sakuma |
| Support Opportunities: | Scholarships abroad - Research Internship - Post-doctor |
| FAPESP's process: | 14/06863-3 - Post-translational modifications in cancer and parasite infection diagnosis: methodological approaches and biological implications |
| Grantee: | Giuseppe Palmisano |
| Support Opportunities: | Research Grants - Young Investigators Grants |
| FAPESP's process: | 15/02866-0 - DISCOVERY AND VERIFICATION OF INTACT GLYCOPEPTIDES AS CANDIDATE PROSTATE CANCER BIOMARKERS USING MASS SPECTROMETRY-BASED PROTEOMICS APPROACHES |
| Grantee: | Rebeca Kawahara Sakuma |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |