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Site-specific characterization of N- and O-linked glycosylation in prostate cancer tissues as molecular signature for disease progression

Grant number: 17/03010-8
Support Opportunities:Scholarships abroad - Research Internship - Post-doctor
Start date: July 10, 2017
End date: July 09, 2018
Field of knowledge:Biological Sciences - Biochemistry - Chemistry of Macromolecules
Principal Investigator:Giuseppe Palmisano
Grantee:Rebeca Kawahara Sakuma
Supervisor: Morten Thaysen Andersen
Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Institution abroad: Macquarie University, Australia  
Associated to the scholarship:15/02866-0 - DISCOVERY AND VERIFICATION OF INTACT GLYCOPEPTIDES AS CANDIDATE PROSTATE CANCER BIOMARKERS USING MASS SPECTROMETRY-BASED PROTEOMICS APPROACHES, BP.PD

Abstract

Prostate cancer (PCa) is the second most common cancer in men worldwide. Gleason score classification is the most important predictor of PCa outcomes and is influential in determining patient treatment options. However, tumor heterogeneity, biopsy-sampling error, and variations in biopsy interpretation are still key challenges for accurate prognostication, leading to significant overtreatment, with associated costs and morbidity. Changings in the glycosylation profile as well as in the glycosylation enzymes were previously described to be associated with prostate cancer detection and aggressiveness and we showed that differentially regulated glycopeptides are able to discriminate urines from prostate cancer and benign conditions (ongoing regular FAPESP project, 2015/02866-0). However, no systematic correlation between altered glycosylation pattern and site-specific changes in protein glycosylation in prostate cancer tissues as prognostic predictor was reported. Therefore, following on the previous work on urine glycoproteins, the objective of this project is to associate site-specific N- and O-linked glycosylation changes with Gleason Score classification, as a strategy to discriminate indolent tumor vs aggressive tumor. For that, quantitative mass spectrometry-based glycoproteomics and glycomics strategies will be performed in human fresh tissues, which will be grouped according to low gleason score (d6), intermediate (7) and high (8-10). Specific glycosylation changes that can potentially discriminate the different Gleason Score groups will be evaluated in larger cohort samples using complementary quantitative strategies. (AU)

News published in Agência FAPESP Newsletter about the scholarship:
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Scientific publications (5)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
CHATTERJEE, SAYANTANI; LEE, LING Y.; KAWAHARA, REBECA; ABRAHAMS, JODIE L.; ADAMCZYK, BARBARA; ANUGRAHAM, MERRINA; ASHWOOD, CHRISTOPHER; SUMER-BAYRAKTAR, ZEYNEP; BRIGGS, MATTHEW T.; CHIK, JENNY H. L.; et al. Protein Paucimannosylation Is an Enriched N-Glycosylation Signature of Human Cancers. PROTEOMICS, v. 19, n. 21-22, SI, . (17/03010-8, 18/15549-1, 15/02866-0, 14/06863-3)
KAWAHARA, REBECA; RECUERO, SAULO; SROUGI, MIGUEL; LEITE, KATIA R. M.; THAYSEN-ANDERSEN, MORTEN; PALMISANO, GIUSEPPE. The Complexity and Dynamics of the Tissue Glycoproteome Associated With Prostate Cancer Progression. MOLECULAR & CELLULAR PROTEOMICS, v. 20, . (14/06863-3, 15/02866-0, 17/03010-8, 18/15549-1, 18/18257-1)
KAWAHARA, REBECA; RECUERO, SAULO; NOGUEIRA, FABIO C. S.; DOMONT, GILBERTO B.; LEITE, KATIA R. M.; SROUGI, MIGUEL; THAYSEN-ANDERSEN, MORTEN; PALMISANO, GIUSEPPE. Tissue Proteome Signatures Associated with Five Grades of Prostate Cancer and Benign Prostatic Hyperplasia. PROTEOMICS, v. 19, n. 21-22, SI, . (18/15549-1, 15/02866-0, 18/18257-1, 17/03010-8, 14/06863-3)
KAWAHARA, REBECA; RECUERO, SAULO; NOGUEIRA, FABIO C. S.; DOMONT, GILBERTO B.; LEITE, KATIA R. M.; SROUGI, MIGUEL; THAYSEN-ANDERSEN, MORTEN; PALMISANO, GIUSEPPE. Tissue Proteome Signatures Associated with Five Grades of Prostate Cancer and Benign Prostatic Hyperplasia. PROTEOMICS, v. 19, n. 21-22, p. 12-pg., . (17/03010-8, 18/18257-1, 18/15549-1, 14/06863-3, 15/02866-0)
CHATTERJEE, SAYANTANI; LEE, LING Y.; KAWAHARA, REBECA; ABRAHAMS, JODIE L.; ADAMCZYK, BARBARA; ANUGRAHAM, MERRINA; ASHWOOD, CHRISTOPHER; SUMER-BAYRAKTAR, ZEYNEP; BRIGGS, MATTHEW T.; CHIK, JENNY H. L.; et al. Protein Paucimannosylation Is an Enriched N-Glycosylation Signature of Human Cancers. PROTEOMICS, v. 19, n. 21-22, p. 13-pg., . (18/15549-1, 17/03010-8, 14/06863-3, 15/02866-0)