| Full text | |
| Author(s): Show less - |
de Sousa, Amanda S.
;
de Melo, Ricardo R.
;
Miyamoto, Renan Y.
;
Morais, Mariana A. B.
;
Andrade, Liliane P.
;
Milan, Natalia
;
de Avila, Mayara C.
;
de Souza, Claudia M.
;
Adao, Regina C.
;
Scarpassa, Josiane A.
;
Vieira, Plinio S.
;
dos Santos, Leandro, V
;
Ramos, Carlos H., I
;
Murakami, Mario T.
;
Zanphorlin, Leticia M.
Total Authors: 15
|
| Document type: | Journal article |
| Source: | BIOFUELS BIOPRODUCTS & BIOREFINING-BIOFPR; v. 14, n. 6, p. 17-pg., 2020-08-20. |
| Abstract | |
The classical route for second-generation ethanol from lignocellulosic biomass is hampered by high process costs, fostering the development of alternative strategies such as simultaneous saccharification and fermentation (SSF). However, the lack of compatible enzyme cocktails poses a challenge. In this study, the enzymeEmBgl from the marine bacteriumExiguobacterium marinumwas rationally identified based on structural and phylogenetic analyses, known desirable properties of close orthologs, and the ecological niche of its organism source.EmBgl is a multifunctional and glucose-tolerant enzyme that efficiently hydrolyzes cello-oligosaccharides due to a positive-subsite region that can accommodate long cello-oligosaccharides without imposing steric impediments. The efficacy ofEmBgl in an SSF process was demonstrated using pretreated sugarcane bagasse as feedstock, yielding 28 g L(-1)of ethanol in 30 h. The distinguishing functional properties ofEmBgl and its successful utilization in an SSF process highlight its potential in biotechnological applications in which lignocellulose deconstruction is desirable under milder temperatures (c) 2020 Society of Chemical Industry and John Wiley & Sons, Ltd. (AU) | |
| FAPESP's process: | 19/08855-1 - New mechanisms of P450: an enzymatic strategy for renewable hydrocarbons |
| Grantee: | Leticia Maria Zanphorlin |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 17/14253-9 - Evaluation of the biotechnological potential of the cellulolytic system of Xanthomonas axonopodis pv. citri: a structural, functional and applied approach |
| Grantee: | Ricardo Rodrigues de Melo |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 18/02865-2 - Molecular investigation of new xylose isomerases for application to lignocellulosic materials fermentation |
| Grantee: | Renan Yuji Miyamoto |
| Support Opportunities: | Scholarships in Brazil - Doctorate (Direct) |
| FAPESP's process: | 15/26982-0 - Exploring novel strategies for depolymerization of plant cell-wall polysaccharides: from structure, function and rational design of glycosyl hydrolases to biological implications and potential biotechnological applications |
| Grantee: | Mário Tyago Murakami |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 16/06509-0 - Understanding the enzymatic system involved in the degradation and utilization of xyloglucans from the plant pathogen Xanthomonas axonopodis pv. citri |
| Grantee: | Plínio Salmazo Vieira |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 16/19995-0 - Analysis of structural and functional diversity of GH43 enzymes from Xanthomonas axonopodis pv. citri: biological implications and potential biotechnological applications |
| Grantee: | Mariana Abrahão Bueno de Morais |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |