Moderate aerobic exercise-induced cytokines changes are disturbed in PPAR alpha knockout mice

Author(s)

Cabral-Santos, Carolina ; Silveira, Loreana Sanches ; Chimin, Patricia ; Rosa-Neto, Jose Cesar ; Lira, Fabio Santos

• Document type: Journal article
• Source: CYTOKINE; v. 134, p. 7-pg., 2020-10-01.

Abstract

The nuclear transcriptional factor peroxisome proliferator activated receptor alpha (PPAR alpha) plays a role in regulating genes involved in lipid metabolism, adipogenesis and inflammation. We aimed to assess the role of PPAR alpha on exercise-mediated locally produced cytokines in adipose fat deposits and skeletal muscle. C57BL/6 (WT) and PPAR alpha knockout (PPAR alpha(-/-)) mice were examined. Each genotype was randomly subdivided into three groups: non-exercised, and euthanized 2 or 24 h after a moderate aerobic exercise session (run on a treadmill at 60% of maximum speed for 1 h). Fat content in gastrocnemius muscle and lipolytic activity in isolated adipose tissue from mesenteric (MEAT) and retroperitoneal (RPAT) adipose tissue were evaluated. In addition, Interleukin 6 (IL-6), interleukin 10 (IL-10), tumor necrosis factor alpha (TNF-alpha) and monocyte chemoat-tractant protein 1 (MCP-1) content were evaluated by ELISA. WT mice showed a maximum lipolysis rate, as well as higher IL-6, IL-10, and IL10/TNF-alpha ratio values 2 h post-exercise (RPAT only) compared with PPAR alpha(-/-)mice. Taken together, our data suggests that PPAR alpha knockout mice exhibited reduced lipolysis and anti-inflammatory response in adipose tissue following exercise, PPAR alpha appears to play an important role in immunomodulatory and lipolysis signaling after acute moderate exercise. (AU)

• FAPESP's process: 18/17648-7 - Acute inflammatory response to moderate-intensity continuous and high-intensity interval training: role to toll-like receptor 4
• Grantee: Carolina Cabral Santos
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• FAPESP's process: 15/26148-0 - Role of toll like receptor 4 (TLR-4) in lipolytic response mediated by acute exhaustive exercise
• Grantee: Carolina Cabral Santos
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 14/01246-6 - Evaluation of immune metabolic response in peritoneal macrophages from obese mice: role of physical exercise and PPAR-gamma
• Grantee: Loreana Sanches Silveira
• Support Opportunities: Scholarships in Brazil - Doctorate