On the release of fluoride from biofilm reservoirs during a cariogenic challenge: anin situstudy

Author(s)

Souza, Joao G. S. ; Del Bel Cury, Altair A. ; Cury, Jaime A. ; Tenuta, Livia M. A.

• Document type: Journal article
• Source: BIOFOULING; v. 36, n. 7, p. 7-pg., 2020-09-29.

Abstract

Biofilm fluoride reservoirs may be a source of fluoride to the fluid phase during a sugar challenge reducing tooth mineral loss. However, the evidence for that is conflicting and has not been studied in biofilms containing different fluoride levels. In order to test fluoride release from biofilms with distinct fluoride concentrations, biofilms were grownin situexposed to a combination of placebo, calcium and fluoride rinses forming biofilms with no (fluoride-free rinses), low (fluoride-only rinses) or high (calcium followed by fluoride rinses) fluoride concentrations, and collected before and 5 min after a sucrose challenge. Rinsing with fluoride increased fluoride concentration in the biofilm (p < 0.05), mainly when a calcium pre-rinse was used before the fluoride (p < 0.05). However, after a sugar challenge, no significant increase in the biofilm fluid fluoride concentration was observed, even in the fluoride-rich biofilms (p > 0.05). Fluoride-rich biofilms do not release fluoride to the fluid phase during a sugar challenge. (AU)

• FAPESP's process: 14/00799-1 - Effect of a calcium prerinse on the anticaries efficacy of fluoridated solution: in situ study
• Grantee: João Gabriel Silva Souza
• Support Opportunities: Scholarships in Brazil - Master