Advances in Recombinant Lipases: Production, Engineering, Immobilization and Application in the Pharmaceutical Industry

Author(s)

Contesini, Fabiano Jares ; Davanco, Marcelo Gomes ; Boriin, Gustavo Pagotto ; Vanegas, Katherina Garcia ; Goncalves Cirino, Joao Pedro ; de Melo, Ricardo Rodrigues ; Mortensen, Uffe Hasbro ; Hilden, Kristiina ; Campos, Daniel Rossi ; Carvalho, Patricia de Oliveira

• Document type: Journal article
• Source: CATALYSTS; v. 10, n. 9, p. 33-pg., 2020-09-01.

Abstract

Lipases are one of the most used enzymes in the pharmaceutical industry due to their efficiency in organic syntheses, mainly in the production of enantiopure drugs. From an industrial viewpoint, the selection of an efficient expression system and host for recombinant lipase production is highly important. The most used hosts areEscherichia coliandKomagataella phaffii(previously known asPichia pastoris) and less often reportedBacillusandAspergillusstrains. The use of efficient expression systems to overproduce homologous or heterologous lipases often require the use of strong promoters and the co-expression of chaperones. Protein engineering techniques, including rational design and directed evolution, are the most reported strategies for improving lipase characteristics. Additionally, lipases can be immobilized in different supports that enable improved properties and enzyme reuse. Here, we review approaches for strain and protein engineering, immobilization and the application of lipases in the pharmaceutical industry. (AU)

• FAPESP's process: 18/13317-6 - A lipidomics study applied to Rectal Cancer: biochemical-molecular mechanisms and assessment of the response to neoadjuvant therapy
• Grantee: Patrícia de Oliveira Carvalho
• Support Opportunities: Regular Research Grants
• FAPESP's process: 17/14253-9 - Evaluation of the biotechnological potential of the cellulolytic system of Xanthomonas axonopodis pv. citri: a structural, functional and applied approach
• Grantee: Ricardo Rodrigues de Melo
• Support Opportunities: Scholarships in Brazil - Post-Doctoral