Susceptibility of Candida albicans and Candida dubliniensis to erythrosine- and LED-mediated photodynamic therapy

Author(s)

Borges Pereira Costa, Anna Carolina ^[1] ; de Campos Rasteiro, Vanessa Maria ^[1] ; Pereira, Cristiane Aparecida ^[1] ; Halter da Silva Hashimoto, Emily Setsuko ^[1] ; Beltrame Junior, Milton ^[2] ; Junqueira, Juliana Campos ^[1] ; Cardoso Jorge, Antonio Olauo ^[1]

Affiliation

^[1] UNESP Univ Estadual Paulista, Dept Biosci & Oral Diag, Sch Dent Sao Jose dos Campos, BR-12245000 Sao Jose Dos Campos, SP - Brazil

^[2] Univ Vale Paraiba UNIVAP, Inst Pesquisa & Desenvolvimento IP&D, BR-12244000 Sao Jose Dos Campos, SP - Brazil

• Document type: Journal article
• Source: ARCHIVES OF ORAL BIOLOGY; v. 56, n. 11, p. 1299-1305, NOV 2011.
• Web of Science Citations: 39

Abstract

The effect of erythrosine- and LED-mediated photodynamic therapy (PDT) on planktonic cultures and biofilms of Candida albicans and Candida dubliniensis was evaluated. Planktonic cultures of standardized suspensions (10(6) cells/mL) of C. albicans and C. dubliniensis were treated with erythrosine concentrations of 0.39-200 mu M and LEDs in a 96-well microtiter plate. Biofilms formed by C. albicans and C. dubliniensis in the bottom of a 96-well microtiter plate were treated with 400 mu M erythrosine and LEDs. After PDT, the biofilms were analysed by scanning electron microscopy (SEM). The antimicrobial effect of PDT against planktonic cultures and biofilms was verified by counting colony-forming units (CFU/mL), and the data were submitted to analysis of variance and the Tukey test (P < 0.05). C. albicans and C. dubliniensis were not detectable after PDT of planktonic cultures with erythrosine concentrations of 3.12 mu M or higher. The CFU/mL values obtained from biofilms were reduced 0.74 log(10) for C. albicans and 0.21 log(10) for C. dubliniensis. SEM revealed a decrease in the quantity of yeasts and hyphae in the biofilm after PDT. In conclusion, C. albicans and C. dubliniensis were susceptible to erythrosine- and LED-mediated PDT, but the biofilms of both Candida species were more resistant than their planktonic counterparts. (C) 2011 Elsevier Ltd. All rights reserved. (AU)

• FAPESP's process: 10/01988-1 - Evaluation of photodynamic therapy on immunosuppressed mice with oral candidosis induced by Candida albicans
• Grantee: Emily Setsuko Halter Hashimoto Miranda
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 09/52048-1 - Effects of photodynamic therapy and essential oils of the Cymbopogon citratus (DC.) Stapf, Casearia sylvestris SW and Curcuma zedoaria Roscoe, in vitro, in biofilms of Candida albicans, Staphylococcus aureus and Streptococcus mutans
• Grantee: Antonio Olavo Cardoso Jorge
• Support Opportunities: Regular Research Grants
• FAPESP's process: 10/18753-7 - Evaluation of photodynamic therapy on Candida albicans in vitro and in vivo
• Grantee: Antonio Olavo Cardoso Jorge
• Support Opportunities: Regular Research Grants
• FAPESP's process: 09/12005-1 - Evaluation of photodynamic therapy in Candida albicans in vitro and in vivo
• Grantee: Anna Carolina Borges Pereira da Costa
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 10/08936-7 - OPPORTUNISTIC MICROORGANISMS IN INJURIES denture stomatitis AND INFLUENCE OF PHOTODYNAMIC THERAPY IN VIRULENCE FACTORS OF Candida spp.
• Grantee: Antonio Olavo Cardoso Jorge
• Support Opportunities: Regular Research Grants
• FAPESP's process: 10/00879-4 - OPPORTUNISTIC MICROORGANISMS IN INJURIES denture stomatitis AND INFLUENCE OF PHOTODYNAMIC THERAPY IN VIRULENCE FACTORS OF Candida spp.
• Grantee: Cristiane Aparecida Pereira Correia
• Support Opportunities: Scholarships in Brazil - Doctorate