Effect of S. mutans combinations with bifidobacteria/lactobacilli on biofilm and enamel demineralization

Author(s)

dos Santos, Vanessa Rodrigues ; Argandona Valdez, Remberto Marcelo ; Danelon, Marcelle ; Santos Souza, Jose Antonio ; Caiaffa, Karina Sampaio ; Botazzo Delbem, Alberto Carlos ; Duque, Cristiane

• Document type: Journal article
• Source: BRAZILIAN ORAL RESEARCH; v. 35, p. 5-pg., 2021-01-01.

Abstract

The present study evaluated the ability of Bifidobacterium and Lactobacillus species associated with streptococci to increase insoluble extracellular polysaccharide (EPS) production and initial caries lesion progression. Bovine enamel blocks (n = 190; 4 mm x 4 mm) were prepared, selected according to initial surface hardness (SH), and divided into two groups: a) double combinations: S. mutans with Bifidobacterium or Lactobacillus, and b) triple combinations: S. mutans and S. sobrinus with Bifidobacterium or Lactobacillus species. The blocks were exposed to the bacterial associations for 7 days. Subsequently, quantity of EPS from biofilms and caries lesion depth were determined by means of colorimetric and cross-sectional enamel hardness (Delta KHN) analysis. The data were submitted to one-way analysis of variance, followed by the Bonferroni test (p < 0.05). S. mutans with B. animalis or B. dentium produced a higher quantity of EPS; S. mutans + B. animalis led to the highest Delta KHN. S. mutans + S. sobrinus + B. longum induced greater EPS and Delta KHN values. In conclusion, associations of B. animalis and B. longum with streptococci promoted EPS production and caries lesion progression. (AU)

• FAPESP's process: 14/02072-1 - Evaluation of in vitro cariogenic potential of bifidobacteria species
• Grantee: Vanessa Rodrigues dos Santos
• Support Opportunities: Scholarships in Brazil - Scientific Initiation