| Full text | |
| Author(s): Show less - |
Oliveira Junior, Gerson A.
;
Pinheiro, Vinicius G.
;
Fonseca, Pablo A. S.
;
Costa, Camila B.
;
Pioltine, Elisa M.
;
Botigelli, Ramon C.
;
Razza, Eduardo M.
;
Ereno, Ronaldo L.
;
Ferraz, Jose B. S.
;
Seneda, Marcelo M.
;
Nogueira, Marcelo F. G.
Total Authors: 11
|
| Document type: | Journal article |
| Source: | LIVESTOCK SCIENCE; v. 249, p. 7-pg., 2021-05-18. |
| Abstract | |
Antral follicle count (AFC) was phenotypically and genetically evaluated in a Bos taurus cow population. A total of 257 Aberdeen Angus cows underwent an ovarian ultrasound evaluation and hormonal synchronization for timed artificial insemination (TAI). These animals were between 40 and 70 d postpartum with a body score condition (BSC) between 2 and 4 (average = 2.7) on a scale of 1-5. The number of antral follicles was assessed 10 d before (D-10) the beginning of TAI. Visible follicles ( 24 follicles and 16 follicles (n = 97); and high AFC as > 25 follicles (n = 67). The comparison between the average AFC and pregnancy rate was performed using the chi-square test in the Minitab (R) 19.2 statistical software, adopting a Pvalue < 0.05. Hair samples of Aberdeen Angus cows were collected for genomic DNA extraction and subsequent genotyping analysis using the GGP HD array. The associations between SNP markers and phenotypic information (AFC) were determined using the GBLUP methodology. Windows explaining more than 1% of the total additive genetic variance were selected as candidates for functional prospection using QTL colocalization and enrichment analysis using a hypergeometric test. The GWAS identified seven candidate genomic windows and 54 annotated genes within them. Among these genes, DUSP1, SOX17, XKR4, PLAG1, HFM1, PPM1K, IBSP, NCAPG-LCORL, and HMGHZ were associated with biological processes that might be related to follicle count in Angus cows. Enrichment analysis of the reproduction-related traits found enriched QTLs in BTA5 ("Inhibin level", "Insulinlike growth factor 1 level"), BTA6 ("Calving ease", "Calf size", "Calving index"), and BTA14 ("Insulin-like growth factor 1 level"; "Age at puberty", "Interval to first estrus after calving"). This study brings new knowledge of the genomic background of AFC and may lead to further investigations regarding physiological explanations for different AFCs and their relationship to fertility. (AU) | |
| FAPESP's process: | 16/16841-2 - Trophectoderm complementation assay for IPS cells: a proposal for assessing pluripotency in cattle |
| Grantee: | Ramon Cesar Botigelli |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 12/50533-2 - GIFT: genomic improvement of fertilization traits in Danish and Brazilian cattle |
| Grantee: | Marcelo Fábio Gouveia Nogueira |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 19/10732-5 - Does the use of natriuretic peptide type-C in in vitro culture of bovine embryos alter the in vitro and in vivo characteristics of its criotolerance? |
| Grantee: | Camila Bortoliero Costa Giovannetti |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 14/26354-6 - Genome-Wide Association Study (GWAS) in reproductive traits of Nellore heifers |
| Grantee: | Gerson Antônio de Oliveira Júnior |
| Support Opportunities: | Scholarships abroad - Research Internship - Doctorate |
| FAPESP's process: | 12/23409-9 - Differential gene expression, ultrastructural analysis and evaluation of the lipid profile of in vitro produced bovine blastocysts from oocytes matured conventionally or via SPOM system |
| Grantee: | Eduardo Montanari Razza |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 14/07566-2 - Genomics applied to ruminant production |
| Grantee: | José Bento Sterman Ferraz |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 13/12097-9 - Genome-wide association studies (GWAS) applied to reproductive traits in Nellore heifers |
| Grantee: | Gerson Antônio de Oliveira Júnior |
| Support Opportunities: | Scholarships in Brazil - Doctorate |