Antimicrobial effect of phytosphingosine in acrylic resin

Author(s)

Luiza Sanchez Pejon ^[1] ; Viviane de Cássia Oliveira ^[2] ; Ayodele Alves Amorim ^[3] ; Júlia Correa Raffaini ^[4] ; Carolina Noronha Ferraz de Arruda ^[5] ; Fernanda de Carvalho Panzeri Pires-de-Souza ^[6]

Affiliation

^[1] University of Sao Paulo. Department of Dental Materials and Prosthodontics Ribeirão Preto School of Dentistry - Brasil

^[2] University of Sao Paulo. Department of Dental Materials and Prosthodontics Ribeirão Preto School of Dentistry - Brasil

^[3] University of Sao Paulo. Department of Dental Materials and Prosthodontics Ribeirão Preto School of Dentistry - Brasil

^[4] University of Sao Paulo. Department of Dental Materials and Prosthodontics Ribeirão Preto School of Dentistry - Brasil

^[5] Rio de Janeiro State University. Department of Prosthodontics of School of Dentistr - Brasil

^[6] University of Sao Paulo. Department of Dental Materials and Prosthodontics Ribeirão Preto School of Dentistry - Brasil

• Document type: Journal article
• Source: Brazilian Dental Journal; v. 34, n. 4, p. 107-114, 2023-10-27.

Abstract

Abstract This study evaluated color stability (CS), anti-adherence effect (AAE), and cell viability of microorganisms on acrylic resin (AR) surface, treated associated or not with sodium percarbonate (SP). AR specimens were prepared, and color analysis was performed before and after the treatments and the CS was calculated. For analysis of AAE, the samples were sterilized by radiation in a microwave oven. Then samples were randomly distributed: phosphate-buffered saline (PBS - control), 0.5% sodium hypochlorite (SH), phytosphingosine (PHS), and phytosphingosine + SP (PHS+Na2CO3). The specimens remained in contact with solutions for 30 minutes and were later contaminated by Candida albicans. Aliquots were seeded in Petri dishes with Sabouraud Dextrose agar and incubated at 37°C for 24 hours. After the incubation, the number of colonies was counted. The cell viability of adhered microorganisms on the AR was evaluated and 20 fields were observed under an epifluorescence microscope, and the percentage of adhered viable cells was calculated. Data were compared (One-way ANOVA, Tukey, p<.05). As for CS, PHS+ Na2CO3 (0.4±0.1) resulted in less change than PBS (0.9±0.2), similar to the other groups (SH [1.0±0.3)]; PHS [0.9±0.2)]). There was no difference for all tested solutions regarding the ability to avoid microorganism adherence (p>0.05), but PHS (11.2±4.1) resulted in a smaller area of adhered viable cells, statistically different from SH (18.2±7.6) and PBS (26.4±10.8). It was concluded that PHS resulted in lower adhered viable cells and when associated with Na2CO3, also shows a lower effect on the CS of AR. (AU)

• FAPESP's process: 20/14021-3 - Antimicrobial effect of phytosphingosine in acrylic resin for complete dentures
• Grantee: Luiza Pejon Sanchez
• Support Opportunities: Scholarships in Brazil - Scientific Initiation