DECREASED EXPRESSION OF MICRORNA-629 IN GASTRIC CANCER SAMPLES POTENTIATED BY THE VIRULENCE MARKER OF H. PYLORI, CAGA GENE

Author(s)

Caroline dos Reis Rodrigues SOARES ^[1] ; Lucas Matheus Vieira da SILVA ^[2] ; Bianca Reis ALMEIDA ^[3] ; Jéssica Nunes PEREIRA ^[4] ; Mônica Pezenatto dos SANTOS ^[5] ; Mônica Santiago BARBOSA ^[6] ; Marília de Arruda Cardoso SMITH ^[7] ; Spencer Luiz Marques PAYÃO ^[8] ; Lucas Trevizani RASMUSSEN

Affiliation

^[1] Faculdade de Medicina de Marília - Brasil

^[2] Faculdade de Medicina de Marília - Brasil

^[3] Faculdade de Medicina de Marília - Brasil

^[4] Faculdade de Medicina de Botucatu - Brasil

^[5] Universidade Estadual de Campinas - Brasil

^[6] Universidade Federal de Goiás - Brasil

^[7] Universidade Federal de São Paulo - Brasil

^[8] Faculdade de Medicina de Marília - Brasil

• Document type: Journal article
• Source: Arq. Gastroenterol.; v. 61, 2024-05-20.

Abstract

ABSTRACT Background: Helicobacter pylori (H. pylori) is a gram-negative bacterium associated with the etiology of several gastrointestinal tract pathologies, and cagA-positive (cagA+) strains are found in populations with gastric ulcers and precancerous lesions, inducing pro-inflammatory responses. The development of neoplasms is related to microRNA (miRNA) dysregulation, indicating highly expressed miRNA-629. The article aims to correlate the expression level of miRNA-629 with the presence of H. pylori and the pathogenicity marker cagA. Methods: 203 gastric biopsy samples were evaluated from individuals with normal gastric tissue (n=60), gastritis (n=96), and gastric cancer (n=47) of both genders and over 18 years old. The samples were subdivided according to the presence or absence of H. pylori, detected by polymerase chain reaction (PCR). RNA was extracted using a commercial kit and quantified. Complementary DNA (cDNA) was synthesized using commercial kits, and the relative expression was calculated using the 2-ΔΔCt method. Results: Individuals infected with H. pylori are nine times more likely to develop gastric cancer. Cancer patients appeared to have decreased expression of miRNA-629; however, the presence of the bacterium would not influence this reduction. Individuals in the cancer group showed lower miRNA-629 expression when cagA+; however, in the control group, the expression was higher when cagA+. Conclusion: H. pylori is a factor involved in the etiology and progression of gastric diseases. Reduction in miRNA-629 expression in cancer patients occurs independent of the presence of the bacterium, but when the cagA pathogenicity marker is present, it induces changes in the gene expression of the respective miRNA. (AU)

• FAPESP's process: 18/02008-2 - Analysis of microRNA expression miR-let7a, miR-125a, miR-155 and the genes of interleukin 6 and its receptor-alpha in patients with gastric diseases and in cell culture: a possible association with H. pylori
• Grantee: Monica Pezenatto dos Santos
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 18/08481-1 - Gene and Protein Interaction among Components of the Inflammatory, Apoptosis and microRNAs Pathways in Gastric Disease and Gastric Cancer
• Grantee: Lucas Trevizani Rasmussen
• Support Opportunities: Regular Research Grants