Unraveling the Nano-Bio Interface Interactions of a Lipase Adsorbed on Gold Nanoparticles under Laser Excitation

Author(s)

de Barros, Heloise Ribeiro ; da Silva, Rafael Trivella Pacheco ; Fernandes, Rafaella ; Toro-Mendoza, Jhoan ; Coluzza, Ivan ; Temperini, Marcia L. A. ; de Torresi, Susana I. Cordoba

• Document type: Journal article
• Source: Langmuir; v. 40, n. 11, p. 10-pg., 2024-03-07.

Abstract

The complex nature and structure of biomolecules and nanoparticles and their interactions make it challenging to achieve a deeper understanding of the dynamics at the nano-bio interface of enzymes and plasmonic nanoparticles subjected to light excitation. In this study, circular dichroism (CD) and Raman spectroscopic experiments and molecular dynamics (MD) simulations were used to investigate the potential changes at the nano-bio interface upon plasmonic excitation. Our data showed that photothermal and thermal heating induced distinct changes in the secondary structure of a model nanobioconjugate composed of lipase fromCandida antarcticafraction B (CALB) and gold nanoparticles (AuNPs). The use of a green laser led to a substantial decrease in the alpha-helix content of the lipase from 66% to 13% and an increase in the beta-sheet content from 5% to 31% compared to the initial conformation of the nanobioconjugate. In contrast, the differences under similar thermal heating conditions were only 55% and 11%, respectively. This study revealed important differences related to the enzyme secondary structure, enzyme-nanoparticle interactions, and the stability of the enzyme catalytic triad (Ser105-Asp187-His224), influenced by the instantaneous local temperature increase generated from photothermal heating compared to the slower rate of thermal heating of the bulk. These results provide valuable insights into the interactions between biomolecules and plasmonic nanoparticles induced by photothermal heating, advancing plasmonic biocatalysis and related fields. (AU)

• FAPESP's process: 17/20892-4 - Nanobioplasmonic catalysts: a novel approach to controlling the selectivity in light-driven one-pot reactions
• Grantee: Heloise Ribeiro de Barros
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 16/21070-5 - Vibrational spectroscopy with spatial resolution
• Grantee: Mauro Carlos Costa Ribeiro
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 18/13492-2 - Synthetic and natural scaffolds applied to regenerative medicine
• Grantee: Luiz Henrique Catalani
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 18/25422-9 - Biofilm synthesis based on lichen beta glucans: study of properties and identification of its vibrational signatures
• Grantee: Rafaella Ferreira Fernandes
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 19/09668-0 - Understanding the biophysiochemical interactions between plasmonic nanoparticles and enzymes to obtain bio-nanomaterials with controlled properties
• Grantee: Heloise Ribeiro de Barros
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
• FAPESP's process: 21/00675-4 - Tying up materials for electrochemical energy storage and catalysis
• Grantee: Roberto Manuel Torresi
• Support Opportunities: Research Projects - Thematic Grants