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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Inflammatory effects of patagonfibrase, a metalloproteinase from Philodryas patagoniensis (Patagonia Green Racer; Dipsadidae) venom

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Author(s):
Elisa Peichoto, Maria [1, 2] ; Zychar, Bianca Cestari [2] ; Luiz Tavares, Flavio [2, 3] ; Goncalves, Luis Roberto Camargo [2] ; Acosta, Ofelia [1] ; Santoro, Marcelo Larami [2]
Total Authors: 6
Affiliation:
[1] Univ Nacl Nordeste, Fac Ciencias Vet, Catedra Farmacol, RA-3400 Corrientes - Argentina
[2] Inst Butantan, Lab Fisiopatol, BR-05503900 Sao Paulo - Brazil
[3] Univ Cuenca Plata, Fac Ingn, Catedra Fisiol, RA-3400 Corrientes - Argentina
Total Affiliations: 3
Document type: Journal article
Source: Experimental Biology and Medicine; v. 236, n. 10, p. 1166-1172, OCT 2011.
Web of Science Citations: 10
Abstract

Patagonfibrase is a P-III class metalloproteinase isolated from the venom of Philodryas patagoniensis, a South-American, rear-fanged `colubrid' snake responsible for accidents with clinical significance. Since local inflammatory reactions are conspicuous signs of snakebites inflicted by this species and taking into consideration that most snake venom metalloproteinases exhibit inflammatory activity, this study deals with the proinflammatory effects evoked by patagonfibrase. Herein, we demonstrate that patagonfibrase causes a time- and dose-dependent hemorrhagic edema when injected into mouse hind paws. The peak of edema occurred at 30 min after injection, and the minimum edematogenic dose was 0.021 mu g. By histological analysis, the presence of moderate to marked edema and hemorrhage, and a mild inflammatory infiltrate was observed. When injected subcutaneously into the scrotal bag of mice, patagonfibrase induced cell recruitment with a significant alteration in physiological parameters of leukocyte-endothelium interaction. The presence of 1 mmol/L o-phenanthroline, which chelates metal ions, significantly inhibited the proinflammatory effects induced by patagonfibrase. Taken together, these results imply that patagonfibrase is an important contributor to local inflammation elicited by P. patagoniensis envenomation, which may pave the way for novel therapeutic strategies to treat this snakebite. Moreover, our findings demonstrate for the first time that a venom metalloproteinase from a rear-fanged snake elicits proinflammatory effects mainly mediated by its catalytic activity. (AU)

FAPESP's process: 08/02996-8 - Venoms from neonate and mature Bothrops jararaca snakes: comparison between enzymatic and biological activities, immunoneutralization, chemical composition and ability to induce hemostatic disorders
Grantee:Marcelo Larami Santoro
Support type: Regular Research Grants
FAPESP's process: 10/08162-1 - Contribution of local injury evoked by Bothrops jararaca snake venom to the genesis of systemic hemostatic disturbances
Grantee:Marcelo Larami Santoro
Support type: Regular Research Grants
FAPESP's process: 10/52559-3 - Importance of local damage induced by snake venom metalloproteinases in the induction of thrombocytopenia in envenomations
Grantee:Marcelo Larami Santoro
Support type: Regular Research Grants