| Full text | |
| Author(s): |
Ito, Lucas T.
;
Miyamoto, Jackson G.
;
Sant'Anna, Savio S.
;
Grego, Kathleen F.
;
Tanaka-Azevedo, Anita M.
;
Tashima, Alexandre K.
Total Authors: 6
|
| Document type: | Journal article |
| Source: | Biochemical and Biophysical Research Communications; v. 683, p. 5-pg., 2023-10-18. |
| Abstract | |
Snake venoms are known to be major sources of peptides with different pharmacological properties. In this study, we comprehensively explored the venom peptidomes of three specimens of Lachesis muta, the largest venomous snake in South America, using mass spectrometry techniques. The analysis revealed 19 main chromatographic peaks common to all specimens. A total of 151 peptides were identified, including 69 from a metalloproteinase, 58 from the BPP-CNP precursor, and 24 from a L-amino acid oxidase. To our knowledge, 126 of these peptides were reported for the first time in this work, including a new SVMP-derived peptide fragment, Lm-10a. Our findings highlight the dynamic nature of toxin maturation in snake venoms, driven by proteolytic processing, post-translational modifications, and cryptide formation. (AU) | |
| FAPESP's process: | 17/21052-0 - Sepsis: mechanisms, therapeutic targets and epidemiology |
| Grantee: | Reinaldo Salomão |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 17/20106-9 - Peptidomics of Brazilian snake and spider venoms |
| Grantee: | Alexandre Keiji Tashima |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 21/11707-4 - Evaluation of alternative in vitro methods to the use of animals for in vivo tests in the study of snake venoms: principle of the 3R's |
| Grantee: | Anita Mitico Tanaka-Azevedo |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 19/07629-8 - Complete sequencing of cysteine rich toxins from the venom of Nephilengys cruentata spider |
| Grantee: | Lucas Toshio Ito |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |