Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Care during freeze-drying of bovine pericardium tissue to be used as a biomaterial: A comparative study

Full text
Author(s):
Polak, Roberta [1] ; Pitombo, Ronaldo N. M. [1]
Total Authors: 2
Affiliation:
[1] Univ Sao Paulo, Sch Pharmaceut Sci, Dept Pharmaceut Technol & Biochem, Sao Paulo - Brazil
Total Affiliations: 1
Document type: Journal article
Source: CRYOBIOLOGY; v. 63, n. 2, p. 61-66, OCT 2011.
Web of Science Citations: 17
Abstract

Bovine pericardium (BP) tissue is widely used in the manufacture of bioprosthetics. The effects of freeze-drying on the BP tissue have been studied by some researchers in order to decrease their cytotoxicity due to preservation in formaldehyde solution, and to increase the lifetime of the product in storage. This study was undertaken in order to study the effect of freeze-drying in the structure of BP. To perform this study BP samples were freeze-dried in two different types of freeze-dryers available in our laboratory: a laboratory freeze-dryer, in which it was not possible to control parameters and a pilot freeze-dryer, wherein all parameters during freezing and drying were controlled. After freeze-drying processes, samples were analyzed by SEM, Raman spectroscopy, tensile strength, water uptake tests and TEM. In summary, it has been demonstrated that damages occur in collagen fibers by the loss of bulk water of collagen structure implicating in a drastic decreasing of BP mechanical properties due to its structural alterations. Moreover, it was proven that the collagen fibrils suffered breakage at some points, which can be attributed to the uncontrolled parameters during drying. (C) 2011 Elsevier Inc. All rights reserved. (AU)