Different modeling approaches for inline biochemical monitoring over the VLP-making upstream stages using Raman spectroscopy

Author(s)

Dias, Vinicius Araga Tejo ; Guardalini, Luis Giovani Oliveira ; Leme, Jaci ; Bernardino, Thaissa Consoni ; da Silveira, Suellen Regina ; Tonso, Aldo ; Jorge, Soraia Attie Calil ; Nunez, Eutimio Gustavo Fernnandez

• Document type: Journal article
• Source: SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY; v. 320, p. 11-pg., 2024-06-15.

Abstract

This work aimed to set inline Raman spectroscopy models to monitor biochemically (viable cell density, cell viability, glucose, lactate, glutamine, glutamate, and ammonium) all upstream stages of a virus -like particlemaking process. Linear (Partial least squares, PLS; Principal components regression, PCR) and nonlinear (Artificial neural networks, ANN; supported vector machine, SVM) modeling approaches were assessed. The nonlinear models, ANN and SVM, were the more suitable models with the lowest absolute errors. The mean absolute error of the best models within the assessed parameter ranges for viable cell density (0.01 -8.83 x 10 6 cells/mL), cell viability (1.3 -100.0 %), glucose (5.22 -10.93 g/L), lactate (18.6 -152.7 mg/L), glutamine (158 -1761 mg/L), glutamate (807.6 -2159.7 mg/L), and ammonium (62.8 -117.8 mg/L) were 1.55 +/- 1.37 x 10 6 cells/mL (ANN), 5.01 +/- 4.93 % (ANN), 0.27 +/- 0.22 g/L (SVM), 4.7 +/- 2.6 mg/L (SVM), 51 +/- 49 mg/L (ANN), 57 +/- 39 mg/L (SVM) and 2.0 +/- 1.8 mg/L (ANN), respectively. The errors achieved, and best -fitted models were like those for the same bioprocess using offline data and others, which utilized inline spectra for mammalian cell lines as a host. (AU)

• FAPESP's process: 22/02713-3 - Establishment of scalable bioprocesses for producing virus-like particles
• Grantee: Eutimio Gustavo Fernández Núñez
• Support Opportunities: Research Grants - Initial Project
• FAPESP's process: 16/22780-6 - Arboviruses VLP production in insect cells.
• Grantee: Soraia Attie Calil Jorge
• Support Opportunities: Regular Research Grants
• FAPESP's process: 18/10538-1 - Characterization of recombinant antigens and immune response of a new rabies vaccine candidate
• Grantee: Renato Mancini Astray
• Support Opportunities: Regular Research Grants
• FAPESP's process: 22/04711-8 - Off-line monitoring of the rabies VLP production bioprocess by Raman spectroscopy
• Grantee: Vinícius Aragão Tejo Dias
• Support Opportunities: Scholarships in Brazil - Scientific Initiation