Enhanced label-free detection of proteins on Au nanoparticle micropatterns for surface-enhanced infrared absorption spectroscopy

Author(s)

Hassan, Ayaz ; de Sousa, Daniel S. ; Bertaglia, Thiago ; Crespilho, Frank N.

• Document type: Journal article
• Source: CHEMICAL COMMUNICATIONS; v. 60, n. 56, p. 4-pg., 2024-06-06.

Abstract

A label-free one-step lithographically masked deposition technique was implemented for the fabrication of gold nanoparticle (Au NP) micropatterns. These micropatterns serve as active substrates for surface-enhanced infrared absorption spectroscopy (SEIRAS) and exhibit a substantial increase in the IR signal upon adsorption of multiple proteins compared to untreated surfaces. Micro-FTIR chemical imaging was conducted to evaluate the efficacy of Au NP micropatterns as singular enhancers for SEIRAS across diverse IR-active substrates demonstrating a promising application for the detection of proteins at low concentrations within biological fluids. An average of 7.5 times enhancement in the IR absorption signal is observed for cytochrome c adsorbed on gold nanoparticle micropatterns as compared to a bare infrared surface in surface enhanced infrared spectroscopy. (AU)

• FAPESP's process: 19/12053-8 - High performance electrodes applied in organic batteries and in biofuel cell
• Grantee: Frank Nelson Crespilho
• Support Opportunities: Regular Research Grants
• FAPESP's process: 19/15333-1 - Bio-photo-electrochemical hybrid cells for solar energy conversion
• Grantee: Frank Nelson Crespilho
• Support Opportunities: Regular Research Grants
• FAPESP's process: 18/22214-6 - Towards a convergence of technologies: from sensing and biosensing to information visualization and machine learning for data analysis in clinical diagnosis
• Grantee: Osvaldo Novais de Oliveira Junior
• Support Opportunities: Research Projects - Thematic Grants