Advanced Extraction Techniques Combined with Natural Deep Eutectic Solvents for Extracting Phenolic Compounds from Pomegranate (Punica granatum L.) Peels

Author(s)

de Oliveira, Isadora Lopes ; Dominguez-Rodriguez, Gloria ; Montero, Lidia ; Vigano, Juliane ; Cifuentes, Alejandro ; Rostagno, Mauricio Arial ; Ibanez, Elena

• Document type: Journal article
• Source: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 25, n. 18, p. 16-pg., 2024-09-01.

Abstract

Pomegranate (Punica granatum L.) peel is a potential source of bioactive phenolic compounds such as ellagic acid and alpha- and beta-punicalagin. This work explores the efficiency of natural deep eutectic solvents combined with ultrasound-assisted extraction (UAE) and pressurized liquid extraction (PLE) for their extraction. Five NaDESs were evaluated by employing UAE (25 degrees C, for 50 min) to determine their total phenolic content (Folin-Ciocalteu assay) and ellagic acid and alpha- and beta-punicalagin contents (high-performance liquid chromatography (HPLC-DAD)). The NaDES composed of choline chloride (ChCl) and glycerol (Gly) (1:2, molar ratio) was the most efficient in the UAE when compared with the rest of the NaDESs and water extracts. Therefore, ChCl:Gly was further evaluated using PLE at different temperatures (40, 80, 120 and 160 degrees C). The PLE-NaDES extract obtained at 80 degrees C for 20 min at 1500 psi exhibited the highest contents of ellagic acid and alpha- and beta-punicalagin compared to the rest of the temperatures and PLE-water extracts obtained under the same extraction conditions. Combining UAE or PLE with a NaDES emerges as a sustainable alternative for extracting ellagic acid and alpha- and beta-punicalagin from pomegranate peel. (AU)

• FAPESP's process: 22/13565-5 - Development of a selective extraction method using pressurized alternative solvents for the fractionation of pomegranate bark compounds (Punica granatum l.)
• Grantee: Isadora Lopes de Oliveira
• Support Opportunities: Scholarships abroad - Research Internship - Master's degree
• FAPESP's process: 18/14582-5 - Development of a two-dimensional extraction, separation and analysis system for evaluating the bioactivity of phytochemicals
• Grantee: Maurício Ariel Rostagno
• Support Opportunities: Research Grants - Young Investigators Grants - Phase 2
• FAPESP's process: 21/13638-0 - Development of active packaging through the extraction of bioactive compounds from pomegranate peel (Punica granatum L.)
• Grantee: Isadora Lopes de Oliveira
• Support Opportunities: Scholarships in Brazil - Master