Chemical composition of strawberries coated with biofilm-based on pomegranate peel extract, chitosan, and gelatin

• Grant number: 19/18748-8
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Start date: August 01, 2020
• End date: December 31, 2023
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
• Principal Investigator: Stanislau Bogusz Junior
• Grantee: Mirella Romanelli Vicente Bertolo
• Host Institution: Instituto de Química de São Carlos (IQSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil
• Associated scholarship(s): 22/04977-8 - Association of agro-industrial waste in the formulation of active chitosan and gelatin-based coatings: valorization of pomegranate and almond peel extracts as sources of phenolic compounds, BE.EP.DD

Abstract

The aim of this research is to evaluate the chemical composition of strawberries covered with edible films made with pomegranate peel extract (Punica granatum L.), chitosan and gelatin. For this, pomegranate peel extracts will be obtained using environmentally friendly solvents such as natural eutectic solvents (NADES). The extracts obtained will have their phenolic components identified and quantified by liquid chromatography coupled with high-resolution mass spectrometry (nanoLC-LTQ-Orbitrap-MS) and liquid chromatography (HPLC-DAD). After chemical characterization, the extract with the highest phenolic content will be incorporated into the biofilms. The ideal concentration of pomegranate extract, chitosan, and gelatin in the films will be obtained through response surface design (mixture modeling) using as the response the results of the rheological, physicochemical, antimicrobial and morphological tests of the materials. Once the coating material composition is optimized, it will be used to coat strawberries, which will be stored under refrigeration. During the storage strawberries will be evaluated: soluble solids content, pH, color, titratable acidity, mass loss, firmness, cellular respiration rate, anthocyanin content and vitamin C. In addition, the volatile profile of the covered strawberries will be evaluated using solid-phase microextraction (SPME) and gas chromatography coupled to mass spectrometry (CG-MS). Finally, sensory tests will be conducted with consumers to know the sensory acceptance, the purchase intent and preference maps. (AU)

Scientific publications

(10)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

EGEA, MARIANA BURANELO; BERTOLO, MIRELLA ROMANELLI VICENTE; OLIVEIRA FILHO, JOSEMAR GONCALVES DE; LEMES, AILTON CESAR. A Narrative Review of the Current Knowledge on Fruit Active Aroma Using Gas Chromatography-Olfactometry (GC-O) Analysis. Molecules, v. 26, n. 17, SEP 2021. (19/18748-8, 18/24612-9)

DE OLIVEIRA FILHO, JOSEMAR GONSALVES; ALBIERO, BEATRIZ REGINA; CALISTO, ITALO HENRIQUE; BERTOLO, MIRELLA ROMANELLI VICENTE; OLDONI, FERNANDA CAMPOS ALENCAR; EGEA, MARIANA BURANELO; BOGUSZ JUNIOR, STANISLAU BOGUSZ; DE AZEREDO, HENRIETTE MONTEIRO CORDEIRO DE; FERREIRA, MARCOS DAVID. Bio-nanocomposite edible coatings based on arrowroot starch/cellulose nanocrystals/carnauba wax nanoemulsion containing essential oils to preserve quality and improve shelf life of strawberry. International Journal of Biological Macromolecules, v. 219, p. 12-pg., 2022-08-12. (18/24612-9, 19/18748-8)

BERTOLO, MIRELLA R. V.; MARTINS, VIRGINIA C. A.; PLEPIS, ANA MARIA G.; BOGUSZ JUNIOR, STANISLAU. Utilization of pomegranate peel waste: Natural deep eutectic solvents as a green strategy to recover valuable phenolic compounds. JOURNAL OF CLEANER PRODUCTION, v. 327, DEC 10 2021. (19/18748-8)

BERTOLO, MIRELLA ROMANELLI VICENTE; DIAS, LUCAS DANILO; FILHO, JOSEMAR GONCALVES DE OLIVEIRA; ALVES, FERNANDA; MARANGON, CRISIANE APARECIDA; AMARO MARTINS, VIRGINIA DA CONCEICA; FERREIRA, MARCOS DAVID; BAGNATO, VANDERLEI SALVADOR; PLEPIS, ANA MARIA DE GUZZI; BOGUSTZ JUNIOR, STANISLAU. Central composite design optimization of active and physical properties of food packaging films based on chitosan/gelatin/pomegranate peel extract. FOOD PACKAGING AND SHELF LIFE, v. 34, p. 11-pg., 2022-11-14. (13/07276-1, 14/50857-8, 19/13569-8, 18/22214-6, 18/24612-9, 19/22799-7, 19/18748-8)

DE OLIVEIRA FILHO, JOSEMAR GONCALVES; VICENTE BERTOLO, MIRELLA ROMANELLI; VIGILATO RODRIGUES, MURILO ALISON; SILVA, GUILHERME DA CRUZ; NAVARRO DE MENDONCA, GIOVANA MARIA; JUNIOR, STANISLAU BOGUSZ; FERREIRA, MARCOS DAVID; EGEA, MARIANA BURANELO. Recent advances in the development of smart, active, and bioactive biodegradable biopolymer-based films containing betalains. Food Chemistry, v. 390, p. 13-pg., 2022-05-13. (18/24612-9, 19/18748-8)

MARANGON, CRISIANE A.; VIGILATO RODRIGUES, MURILO A.; VICENTE BERTOLO, MIRELLA R.; AMARO MARTINS, VIRGINIA DA C.; DE GUZZI PLEPIS, ANA MARIA; NITSCHKE, MARCIA. The effects of ionic strength and pH on antibacterial activity of hybrid biosurfactant-biopolymer nanoparticles. Journal of Applied Polymer Science, v. 139, n. 1, JUL 2021. (19/14405-9, 19/18748-8)

DE ALMEIDA, LARISSA CHIRINO; BERTOLO, MIRELLA ROMANELLI VICENTE; CARDOSO, DANIEL RODRIGUES; BOGUSZ JR, STANISLAU. Effect of Hop beta-Acids Extract Supplementation on the Volatile Compound Profile of Roasted Chicken Meat. PROCESSES, v. 11, n. 1, p. 13-pg., 2023-01-01. (19/18748-8, 17/01189-0)

DE OLIVEIRA FILHO, JOSEMAR GONCALVES; VICENTE BERTOLO, MIRELLA ROMANELLI; BRITO, SABRINA DA COSTA; DONIZETTE MALAFATTI, JOAO OTAVIO; BERTAZZO, GIOVANA BRAIT; COLACIQUE, MATHEUS NUNES; PARIS, ELAINE CRISTINA; BOGUSZ JUNIOR, STANISLAU; FERREIRA, MARCOS DAVID. Recent Advances in the Application of Nanotechnology to Reduce Fruit and Vegetable Losses During Post-Harvest. Brazilian Journal of Physics, v. 52, n. 4, p. 11-pg., 2022-08-01. (19/18748-8, 18/24612-9)

DIAS, LUCAS D.; BERTOLO, MIRELLA ROMANELLI, V; ALVES, FERNANDA; DE FARIA, CLARA M. G.; RODRIGUES, MURILO ALISON, V; LOPES, LETICIA KELLER B. C.; DE GUZZI PLEPIS, ANA MARIA; MATTOSO, LUIZ HENRIQUE C.; BOGUSZ JUNIOR, STANISLAU; BAGNATO, VANDERLEI S.. Preparation and characterization of curcumin and pomegranate peel extract chitosan/gelatin-based Films and their photoinactivation of bacteria. MATERIALS TODAY COMMUNICATIONS, v. 31, p. 10-pg., 2022-06-01. (19/18748-8, 14/50857-8, 19/13569-8)

BERTOLO, MIRELLA ROMANELLI VICENTE; MARTINS, VIRGINIA DA NCEICAO AMARO; PLEPIS, ANA MARIA DE GUZZI; BOGUSZ JR, STANISLAU. Acerola (Malpighia emarginata) and açaí (Euterpe oleracea) extracts as active compounds of starch/gelatin-based solutions: Rheological characterization. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, v. 676, p. 10-pg., 2023-11-05. (19/18748-8)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

BERTOLO, Mirella Romanelli Vicente. Development and application of new polymeric materials based on chitosan, gelatin, and pomegranate (Punica Granatum L.) peel extract for coating and improving the post-harvest quality of strawberries (Fragaria × ananassa Duch.). 2024. Doctoral Thesis - Universidade de São Paulo (USP). Instituto de Química de São Carlos (IQSC/BT) São Carlos.