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Chemical composition of strawberries coated with biofilm-based on pomegranate peel extract, chitosan, and gelatin

Grant number: 19/18748-8
Support type:Scholarships in Brazil - Doctorate (Direct)
Effective date (Start): August 01, 2020
Effective date (End): February 29, 2024
Field of knowledge:Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
Principal researcher:Stanislau Bogusz Junior
Grantee:Mirella Romanelli Vicente Bertolo
Home Institution: Instituto de Química de São Carlos (IQSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil


The aim of this research is to evaluate the chemical composition of strawberries covered with edible films made with pomegranate peel extract (Punica granatum L.), chitosan and gelatin. For this, pomegranate peel extracts will be obtained using environmentally friendly solvents such as natural eutectic solvents (NADES). The extracts obtained will have their phenolic components identified and quantified by liquid chromatography coupled with high-resolution mass spectrometry (nanoLC-LTQ-Orbitrap-MS) and liquid chromatography (HPLC-DAD). After chemical characterization, the extract with the highest phenolic content will be incorporated into the biofilms. The ideal concentration of pomegranate extract, chitosan, and gelatin in the films will be obtained through response surface design (mixture modeling) using as the response the results of the rheological, physicochemical, antimicrobial and morphological tests of the materials. Once the coating material composition is optimized, it will be used to coat strawberries, which will be stored under refrigeration. During the storage strawberries will be evaluated: soluble solids content, pH, color, titratable acidity, mass loss, firmness, cellular respiration rate, anthocyanin content and vitamin C. In addition, the volatile profile of the covered strawberries will be evaluated using solid-phase microextraction (SPME) and gas chromatography coupled to mass spectrometry (CG-MS). Finally, sensory tests will be conducted with consumers to know the sensory acceptance, the purchase intent and preference maps. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
EGEA, MARIANA BURANELO; BERTOLO, MIRELLA ROMANELLI VICENTE; OLIVEIRA FILHO, JOSEMAR GONCALVES DE; LEMES, AILTON CESAR. A Narrative Review of the Current Knowledge on Fruit Active Aroma Using Gas Chromatography-Olfactometry (GC-O) Analysis. Molecules, v. 26, n. 17 SEP 2021. Web of Science Citations: 0.
MARANGON, CRISIANE A.; VIGILATO RODRIGUES, MURILO A.; VICENTE BERTOLO, MIRELLA R.; AMARO MARTINS, VIRGINIA DA C.; DE GUZZI PLEPIS, ANA MARIA; NITSCHKE, MARCIA. The effects of ionic strength and pH on antibacterial activity of hybrid biosurfactant-biopolymer nanoparticles. Journal of Applied Polymer Science, JUL 2021. Web of Science Citations: 0.

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