Leishmanial activity of Brazilian brown propolis and its diterpenes

Author(s)

Oliveira, Larissa Costa ; Ribeiro, Victor Pena ; Santos, Mario Ferreira Conceicao ; Oliveira, Nicoli Dias ; Zago, Matheus Henrique Marques ; Albernaz, Iaciara Luana de Xavier ; Veneziani, Rodrigo Cassio Sola ; Bastos, Jairo Kenupp ; Magalhaes, Lizandra Guidi ; Ambrosio, Sergio Ricardo

• Document type: Journal article
• Source: NATURAL PRODUCT RESEARCH; v. 38, n. 23, p. 5-pg., 2023-10-30.

Abstract

Propolis is a natural product widely used in folk medicine. Among its various applications, its antiparasitic properties stand out. Due to its great biodiversity, Brazil is a major producer of several types of propolis. This study proposes to evaluate the leishmanicidal properties of the hydroalcoholic extract of propolis collected in the southern region of Brazil (Brown propolis - HEBP) and its main isolated compounds: abietic acid (1), 13-epi-cupressic acid (2), 13-epi-torulosol (3), dehydroabietic acid (4), cis-communic acid (5) and ent-agatic acid (6). In general, the diterpenes did not show activity against the promastigotes of Leishmania (Leishmania) amazonensis at the evaluated concentrations. However, the HEBP was very active with an inhibition concentration of 50% at 8.32 mu g/mL. Moreover, transmission electron microscopy (TEM) and scanning electron microscopy (SEM) assays showed morphological and structural alterations in promastigote forms of L. (L.) amazonensis when incubated with HEBP. (AU)

• FAPESP's process: 22/05874-8 - In vivo evaluation of the combination of a standardized extract of Brazilian Red Propolis with Praziquantel in the murine model of schistosomiasis mansoni.
• Grantee: Matheus Henrique Marques Zago
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 17/04138-8 - Attainment of chemical, analytical, biological, pharmacological and technological studies to fill the gaps on the development of Brazilian propolis sector
• Grantee: Jairo Kenupp Bastos
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 22/00659-1 - In vitro evaluation of the metabolism of the major compounds of Araucaria sp brown propolis
• Grantee: Victor Pena Ribeiro
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 18/05080-6 - Multi-user equipment approved in grant 2017/04138-8 for high pressure semipreparative chromatographic system model LC-20AR
• Grantee: Sergio Ricardo Ambrosio
• Support Opportunities: Multi-user Equipment Program