D-limonene supplementation does not alter postprandial metabolism of postmenopausal women challenged with a mixed macronutrient tolerance test: a pilot study

Author(s):Show less -

Vasconcelos, Amanda D. ; Donado-Pestana, Carlos M. ; More, Tushar H. ; Duarte, Graziela B. S. ; Duarte, Stephany G. ; Dias, Caroline G. ; Rodrigues, Larissa ; Hernandez, Guilherme N. ; Fock, Ricardo ; Hiller, Karsten ; Fiamoncini, Jarlei

• Document type: Journal article
• Source: FOOD PRODUCTION PROCESSING AND NUTRITION; v. 6, n. 1, p. 12-pg., 2024-07-01.

Abstract

The hormonal decline during menopause increases women's risk of chronic diseases. D-limonene, a monoterpene found in the human diet, possesses biological properties related to hypolipemic, antioxidant, anti-inflammatory, and gut microbiota-modulating activities, primarily observed in preclinical studies. Postprandial responses include physiological adaptations to the stress of a nutrient surplus, providing an opportunity to assess metabolic resilience, being a suitable strategy for exploring post-menopause-associated metabolic alterations. Here, we investigated the effects of D-limonene supplementation (2 g/day) on postprandial metabolism in postmenopausal women (n = 9) challenged with a standardized mixed meal in a 3-week single-arm clinical study. Our findings revealed that D-limonene did not induce marked differences in postprandial responses to the dietary challenge. The supplementation with D-limonene induced no alterations in serum lipid/lipoprotein profile or glycemia/insulinemia. D-limonene supplementation did not affect the transient postprandial inflammatory response regarding changes in gene expression of peripheral blood mononuclear cells (PBMC) and circulating inflammatory markers. Nevertheless, D-limonene reduced postprandial levels of lithocholic acid, a gut microbiota-derived bile acid, and regulated the plasma concentrations of selected amino acids, carbohydrate metabolism-derived metabolites, and organic acids. In conclusion, our data do not support the claim that short-term D-limonene supplementation beneficially affects the postprandial metabolism of postmenopausal women. (AU)

• FAPESP's process: 13/07914-8 - FoRC - Food Research Center
• Grantee: Bernadette Dora Gombossy de Melo Franco
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
• FAPESP's process: 19/24311-1 - Study of d-limonene effects on plasma markers of lipid and glucose metabolism in postmenopausal women
• Grantee: Amanda Domingos Vasconcelos
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 20/16542-0 - Relationship between inflammation and primary metabolism in the postprandial period
• Grantee: Carlos Mario Donado Pestana
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 22/08109-0 - Evaluation of the aging effects on postprandial responses: comparison between reproductive-age and postmenopausal women.
• Grantee: Amanda Domingos Vasconcelos
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 22/02941-6 - Bile acids as determinants of postprandial metabolism and intestinal microbial colonization in newborns
• Grantee: Jarlei Fiamoncini
• Support Opportunities: Research Grants - Initial Project
• FAPESP's process: 21/08657-5 - Evaluation of D-limonene effects on postprandial intermediary metabolism using "dry blood spots" as a sampling tool in a clinical study carried out remotely
• Grantee: Jarlei Fiamoncini
• Support Opportunities: Regular Research Grants