Interesterified palm oil promotes insulin resistance and altered insulin secretion and signaling in Swiss mice

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dos Santos, Raisa Magno ; Miyamoto, Josiane Erica ; Siqueira, Beatriz Piatezzi ; Araujo, Thiago Reis ; Vettorazzi, Jean Franciesco ; Menta, Penelope Lacrisio Reis ; Denom, Jessica ; Latorraca, Marcia Queiroz ; Cruciani-Guglielmacci, Celine ; Carneiro, Everardo Magalhaes ; Torsoni, Adriana ; Torsoni, Marcio ; Badan, Ana Paula ; Magnan, Christophe ; Le Stunff, Herve ; Ignacio-Souza, Leticia ; Milanski, Marciane

• Document type: Journal article
• Source: Food Research International; v. 177, p. 12-pg., 2023-12-21.

Abstract

Interesterified fats have been used to replace trans-fat in ultra-processed foods. However, their metabolic effects are not completely understood. Hence, this study aimed to investigate the effects related to glucose homeostasis in response to interesterified palm oil or refined palm oil intake. Four-week-old male Swiss mice were randomly divided into four experimental groups and fed the following diets for 8 weeks: a normocaloric and normolipidic diet containing refined palm oil (PO group) or interesterified palm oil (IPO group); a hypercaloric and high-fat diet containing refined PO (POHF group) or interesterified PO (IPOHF group). Metabolic parameters related to body mass, adiposity and food consumption showed no significant differences. As for glucose homeostasis parameters, interesterified palm oil diets (IPO and IPOHF) resulted in higher glucose intolerance than unmodified palm oil diets (PO and POHF). Euglycemic-hyperinsulinemic clamp assessment showed a higher endogenous glucose production in the IPO group compared with the PO group. Moreover, the IPO group showed significantly lower p-AKT protein content (in the muscle and liver tissues) when compared with the PO group. Analysis of glucose-stimulated static insulin secretion (11.1 mmol/L glucose) in isolated pancreatic islets showed a higher insulin secretion in animals fed interesterified fat diets (IPO and IPOHF) than in those fed with palm oil (PO and POHF). Interesterified palm oil, including in normolipidic diets, can impair insulin signaling in peripheral tissues and increase insulin secretion by beta-cells, characterizing insulin resistance in mice. (AU)

• FAPESP's process: 20/16040-5 - Metabolic effects of interesterified fat and its metabolites on glucose and lipid homeostasis
• Grantee: Josiane Érica Miyamoto
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• FAPESP's process: 16/24768-3 - Evaluation of metabolic parameters of experimental animal models in response to dietary interesterified fat: underlaying molecular mechanisms
• Grantee: Josiane Érica Miyamoto
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 19/26538-3 - Effects of intersterified fat on the intestinal permeability of healthy and obese animals
• Grantee: Marciane Milanski Ferreira
• Support Opportunities: Regular Research Grants