Label- and Redox Probe-Free Bioelectronic Chip for Monitoring Vitamins C and the 25-Hydroxyvitamin D3 Metabolite

Author(s)

Martins, Thiago S. ; Bott-Neto, Jose L. ; Oliveira Jr, Osvaldo N.

• Document type: Journal article
• Source: ACS APPLIED NANO MATERIALS; v. 7, n. 5, p. 8-pg., 2024-01-27.

Abstract

Monitoring immune-supporting micronutrients such as vitamins C and D is relevant as these nutrients are involved in metabolic pathways to fight against viruses and bacteria. The simultaneous determination of these biomarkers in the same sample volume is challenging, with multistep protocols if labels and redox probes are required. In this paper, we report on a flexible bioelectronic chip combining a 25-hydroxyvitamin D3 metabolite (25(OH)D3) immunoassay with an electrocatalytic assay for vitamin C. One of the sensors in the chip was made with graphitic carbon nitride modified with the electroactive dye toluidine blue, decorated with electrodeposited 6.5 nm gold nanoparticles, and coated with a layer of anti-25(OH)D3 antibodies. With this sensor, 25(OH)D3 could be detected via chronoamperometry at concentrations ranging from 0.1 to 700 ng mL(-1) with a limit of detection (LOD) of 0.01 ng mL(-1). The other sensor had 24.7 nm Pearls carbon nanoparticles whose electroactivity permitted vitamin C detection in the range from 1 to 140 mu M, with an LOD of 0.12 mu M. Since the chip is flexible and does not contain labels or redox probes, it has the potential for use in wearable devices to track vitamins in bodily fluids, aiding personalized nutrition by preventing deficiencies and toxicity. (AU)

• FAPESP's process: 22/03758-0 - Integrated dual electrochemical device for multiplexed detection of biomarkers
• Grantee: Thiago Serafim Martins
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 19/13514-9 - Electrochemical sensors with a matrix containing graphene quantum dots or nanoribbons for the detection of biomarkers
• Grantee: Jose Luiz Bott Neto
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 22/15122-3 - Developing new breathable tattoo (bio)sensors for health monitoring
• Grantee: Thiago Serafim Martins
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
• FAPESP's process: 18/22214-6 - Towards a convergence of technologies: from sensing and biosensing to information visualization and machine learning for data analysis in clinical diagnosis
• Grantee: Osvaldo Novais de Oliveira Junior
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 22/00243-0 - Developing new components and processes in visible light activated biosensors
• Grantee: Jose Luiz Bott Neto
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor