Insights into the global genomic features of Salmonella enterica serovar Gallinarum biovars Gallinarum and Pullorum

Author(s)

Campos, Isabela C. ; Vilela, Felipe Pinheiro ; Saraiva, Mauro de M. S. ; Junior, Angelo Berchieri ; Falcao, Juliana Pfrimer

• Document type: Journal article
• Source: Journal of Applied Microbiology; v. 135, n. 9, p. 11-pg., 2024-09-02.

Abstract

Aims Characterize global genomic features of 86 genomes of Salmonella Gallinarum (SG) and Pullorum (SP), which are important pathogens causing systemic infections in poultry.Methods and results All genomes harbored efflux pump encoding gene mdsA and gold tolerance genes golS and golT. Aminoglycoside (aac(6 ')-Ib, aadA5, aph(6)-Id, aph(3 '')-Ib, ant(2 '')-Ia), beta-lactam (blaTEM-1, blaTEM-135), efflux pump (mdsB), fosfomycin (fosA3), sulfonamide (sul1, sul2), tetracycline [tet(A)], trimethoprim (dfrA17), acid (asr), and disinfectant (qacEdelta1) resistance genes, gyrA, gyrB, and parC quinolone resistance point mutations, and mercury tolerance genes (mer) were found in different frequencies. Additionally, 310 virulence genes, pathogenicity islands (including SPI-1, 2, 3, 4, 5, 6, 9, 10, 12, 13, and 14), plasmids [IncFII(S), ColpVC, IncX1, IncN, IncX2, and IncC], and prophages (Fels-2, ST104, 500465-1, pro483, Gifsy-2, 103 203_sal5, Fels-1, RE-2010, vB_SenS-Ent2, and L-413C) were detected. MLST showed biovar-specific sequence types, and core genome MLST showed country-specific and global-related clusters.Conclusion SG and SP global strains carry many virulence factors and important antimicrobial resistance genes. The diverse plasmids and prophages suggest genetic variability. MLST and cgMLST differentiated biovars and showed profiles occurring locally or worldwide. (AU)

• FAPESP's process: 21/07365-0 - Genomic, transcriptomic and phenotypic evaluation of virulence aspects of Salmonella Infantis strains isolated from diverse sources in Brazil
• Grantee: Felipe Pinheiro Vilela
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 22/07013-0 - Genome analyses, transcriptome, mutants' construction and phenotypic characterization of Campylobacter coli strains isolated from different sources during 25 years in Brazil
• Grantee: Juliana Pfrimer Falcão
• Support Opportunities: Regular Research Grants