Optimized Enzymatic Synthesis of Hesperidin Fatty Acid Esters in a Two-Phase System Containing Ionic Liquid

Author(s)

Melo Branco de Araujo, Maria Elisa ^[1] ; Contesini, Fabiano Jares ^[2] ; Moreira Franco, Yollanda Edwirges ^[1] ; Frankland Sawaya, Alexandra C. H. ^[3] ; Alberto, Thiago Grando ^[1] ; Dalfre, Natalia ^[1] ; Carvalho, Patricia de Oliveira ^[1]

Affiliation

^[1] Univ Sao Francisco, BR-12916900 Braganca Paulista, SP - Brazil

^[2] State Univ Campinas UNICAMP, Fac Food Engn, Dept Food Sci, Lab Food Biochem, BR-13083862 Campinas, SP - Brazil

^[3] State Univ Campinas UNICAMP, Inst Biol, Dept Plant Biol, BR-13083970 Campinas, SP - Brazil

• Document type: Journal article
• Source: Molecules; v. 16, n. 8, p. 7171-7182, AUG 2011.
• Web of Science Citations: 7

Abstract

Response surface methodology (RSM) based on a five-level, three-variable central composite design (CCD) was employed for modeling and optimizing the conversion yield of the enzymatic acylation of hesperidin with decanoic acid using immobilized Candida antarctica lipase B (CALB) in a two-phase system containing {[}bmim]BF(4). The three variables studied (molar ratio of hesperidin to decanoic acid, {[}bmim]BF(4)/acetone ratio and lipase concentration) significantly affected the conversion yield of acylated hesperidin derivative. Verification experiments confirmed the validity of the predicted model. The lipase showed higher conversion degree in a two-phase system using {[}bmim] BF4 and acetone compared to that in pure acetone. Under the optimal reaction conditions carried out in a single-step biocatalytic process when the water content was kept lower than 200 ppm, the maximum acylation yield was 53.6%. (AU)

• FAPESP's process: 09/09224-3 - Biotransformation and antioxidant properties of polyphenols
• Grantee: Patrícia de Oliveira Carvalho
• Support Opportunities: Regular Research Grants