| Full text | |
| Author(s): |
Bauermeister, Anelize
;
Furtado, Luciana Costa
;
Ferreira, Elthon G.
;
Moreira, Eduarda Antunes
;
Jimenez, Paula Christine
;
Lopes, Norberto Peporine
;
Araujo, Welington Luiz
;
Olchanheski, Luiz Ricardo
;
Lotufo, Tito Monteiro da Cruz
;
Costa-Lotufo, Leticia Veras
Total Authors: 10
|
| Document type: | Journal article |
| Source: | MARINE ENVIRONMENTAL RESEARCH; v. 194, p. 13-pg., 2023-12-26. |
| Abstract | |
The tropical ascidian Eudistoma vannamei, endemic to the northeastern coast of Brazil, is considered a prolific source of secondary metabolites and hosts Actinomycetota that produce bioactive compounds. Herein, we used an omics approach to study the ascidian as a holobiont, including the microbial diversity through 16S rRNA gene sequencing and metabolite production using mass spectrometry-based metabolomics. Gene sequencing analysis revealed all samples of E. vannamei shared about 50% of the observed ASVs, and Pseudomonadota (50.7%), Planctomycetota (9.58%), Actinomycetota (10.34%), Bacteroidota (12.05%) were the most abundant bacterial phyla. Analysis of tandem mass spectrometry (MS/MS) data allowed annotation of compounds, including phospholipids, amino acids, and pyrimidine alkaloids, such as staurosporine, a member of a well-known chemical class recognized as a microbial metabolite. Isolated bacteria, mainly belonging to Streptomyces and Micromonospora genera, were cultivated and extracted with ethyl acetate. MS/MS analysis of bacterial extracts allowed annotation of compounds not detected in the ascidian tissue, including marineosin and dihydroergot-amine, yielding about 30% overlapped ions between host and isolated bacteria. This study reveals E. vannamei as a rich source of microbial and chemical diversity and, furthermore, highlights the importance of omic tools for a comprehensive investigation of holobiont systems. (AU) | |
| FAPESP's process: | 20/02207-5 - Inventorying secondary metabolism applying metabolomic strategies: contribution to the Brazilian biodiversity valuation |
| Grantee: | Norberto Peporine Lopes |
| Support Opportunities: | BIOTA-FAPESP Program - Thematic Grants |
| FAPESP's process: | 19/03008-9 - Chemical investigation on marine actinomycetes extracts with potential cytotoxic activity in tumoral lines and insecticide activity on Aedes aegypti species |
| Grantee: | Eduarda Antunes Moreira |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 15/17177-6 - Integrative approach on the sustainable prospection of marine natural products: from diversity to anticancer compounds |
| Grantee: | Leticia Veras Costa Lotufo |
| Support Opportunities: | BIOTA-FAPESP Program - Thematic Grants |
| FAPESP's process: | 22/12654-4 - Marine myxobacteria from the state of São Paulo as a source of protease inhibitors |
| Grantee: | Paula Christine Jimenez |
| Support Opportunities: | BIOTA-FAPESP Program - Regular Research Grants |
| FAPESP's process: | 17/18235-5 - Anticancer potential of isolated substances from Streptomyces SP. recovered from ascidian Euherdmania SP |
| Grantee: | Luciana Costa Furtado |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 20/08987-2 - Effect of histone deacetylase and proteasome inhibitors in Glioma models |
| Grantee: | Luciana Costa Furtado |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 17/17648-4 - Integrative approach on the sustainable prospection of marine natural products: from diversity to anticancer compounds |
| Grantee: | Anelize Bauermeister |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |