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Spectroscopy Technologies to Screen Peanut Seeds with Superior Vigor Through "Chemical Fingerprinting"

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Author(s):
de Oliveira, Gustavo Roberto Fonseca ; Hirai, Welinton Yoshio ; Ferreira, Dennis Silva ; da Silva, Karolyne Priscila Oliveira Mota ; Silva, Giovani Chaves ; Moraes, Tiago Bueno ; Mastrangelo, Clissia Barboza ; Pereira, Fabiola Manhas Verbi ; Pereira-Filho, Edenir Rodrigues ; da Silva, Edvaldo Aparecido Amaral
Total Authors: 10
Document type: Journal article
Source: AGRONOMY-BASEL; v. 14, n. 11, p. 17-pg., 2024-11-01.
Abstract

Peanut seeds are harvested at different development stages (early and late) due to their uneven maturation. At the time of harvest, approximately 30% of the seeds are still immature, meaning they are not completely filled with compounds (e.g., oil and minerals) and exhibit reduced vigor. Hypothetically, these compounds can be detected as a "chemical fingerprinting" to classify seed maturation stages. Here, we investigated whether non-destructive techniques such as benchtop nuclear magnetic resonance (NMR), laser-induced breakdown spectroscopy (LIBS), and energy-dispersive X-ray fluorescence (ED-XRF) can identify chemical patterns unique to mature seeds with superior vigor. Field-grown seeds were classified into early (R5 and R6) and late (R7, R8, and R9) stages. Seed weight, germination, vigor, H2O2, and MDA (oxidative stress) were analyzed. Oil, potassium (K), and calcium (Ca) were measured digitally using spectroscopy techniques. We found that: (i) oxidative stress and K levels were higher in seeds from the early stages; (ii) seed oil and Ca were proportional to high-vigor seedlings and successful plant establishment in the field; and (iii) the seed chemical composition could be identified autonomously with 87% to 100% accuracy. In conclusion, LIBS, ED-XRF, and NMR technologies can effectively screen peanut seeds with superior vigor through "chemical fingerprinting". (AU)

FAPESP's process: 20/14050-3 - Peanut seed maturity disuniformity: physiological, chemical and transcriptomic approach
Grantee:Gustavo Roberto Fonseca de Oliveira
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 14/50945-4 - INCT 2014: National Institute for Alternative Technologies of Detection, Toxicological Evaluation and Removal of Micropollutants and Radioactivies
Grantee:Maria Valnice Boldrin
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 19/01102-8 - Multi-user Equipament approved in grant 2018/18212-8: X-ray fluorescence spectrometer
Grantee:Fabiola Manhas Verbi Pereira
Support Opportunities: Multi-user Equipment Program
FAPESP's process: 21/10882-7 - Non-conventional food plants (PANC) - accessible, functional, and sustainable nutritional source
Grantee:Fabiola Manhas Verbi Pereira
Support Opportunities: Regular Research Grants
FAPESP's process: 23/00435-9 - Anthocyanin: a new biomarker for physiological quality of peanut seeds?
Grantee:Gustavo Roberto Fonseca de Oliveira
Support Opportunities: Scholarships abroad - Research Internship - Doctorate
FAPESP's process: 22/02232-5 - No-invasive characterization of unconventional food plants (PANC) by using energy-dispersive X-ray fluorescence and chemometrics
Grantee:Leticia da Silva Rodrigues
Support Opportunities: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 21/07331-9 - Physiological and molecular studies during the acquisition of longevity in peanut seeds (Arachis hypogaea L.)
Grantee:Edvaldo Aparecido Amaral da Silva
Support Opportunities: Regular Research Grants