Risk factors and immunological biomarkers in denture stomatitis: An observational cross-sectional study

Author(s)

Clemente, Lorena Mosconi ; Ribeiro, Adriana Barbosa ; Fortes, Caroline Vieira ; Ribeiro, Aline Barbosa ; Oliveira, Viviane de Cassia ; Macedo, Ana Paula ; Salgado, Helio Cesar ; da Silva, Claudia Helena Lovato

• Document type: Journal article
• Source: ARCHIVES OF ORAL BIOLOGY; v. 155, p. 9-pg., 2023-09-04.

Abstract

Objective: Assess risk factors, local and systemic immunological biomarkers in healthy individuals and with Denture Stomatitis (DS). Design: For this observational transversal study, 27 participants without DS (Group 0), 24 with moderate DS (Group 1), and 25 with severe DS (Group 2) were assessed for sociodemographic, behavioral, and clinical parameters, microbial load of Candida spp., Staphylococcus spp., Streptococcus mutans, Pseudomonas spp., and enterobacteria, and cytokine and C-reactive protein levels. ANOVA, Fisher's exact, Kruskal-Wallis, Mann-Whitney, Wilcoxon and Pearson's chi-square tests were used for data analysis (alpha = 0.05). Results: Group 1 had a significantly higher mean age compared to the other groups (P = 0.018), but no correlation was identified between age and DS (P = 0.830; r = 0.025). No significant differences were found among the groups for other sociodemographic and behavioral characteristics. Group 1 had significantly older upper and lower dentures; however, no correlation was identified between age of upper (P = 0.522; r = 0.075) and lower (P = 0.143; r = 0.195) dentures and DS. The microbial load of Candida albicans on the dentures (P = 0.035) and Candida spp. on the palate (P = 0.008) of the groups 1 and 2 was higher than group 0. Group 1 and 2 had higher Candida spp. counts on denture (P = 0.003) than group 0. There was no difference among groups for bacterial analyzed. Group 1 showed higher and Group 2 intermediate salivary levels of IL-6 compared to Group 0. There was no difference in the C-reactive protein levels among groups. Conclusions: Microbial load of Candida spp. is the factor with the strongest relationship with DS, with capacity for local signaling through IL-6. (AU)

• FAPESP's process: 20/06043-7 - Autonomic (sympathetic and parasympathetic) modulation of cardiovascular and inflammatory responses under physiopathological clinical and experimental conditions
• Grantee: Helio Cesar Salgado
• Support Opportunities: Research Projects - Thematic Grants