| Full text | |
| Author(s): |
Maimone, Naydja M.
;
Pereira, Alana K.
;
Gimenes, Leila
;
Fernandes, Hocelayne P.
;
Fill, Taicia P.
;
Borges, Ricardo M.
;
Lira, Simone P.
;
Fernandes, Joao B.
;
Bauermeister, Anelize
Total Authors: 9
|
| Document type: | Journal article |
| Source: | REVISTA VIRTUAL DE QUIMICA; v. 16, n. 2, p. 23-pg., 2023-11-24. |
| Abstract | |
The incredible natural diversity that we can admire at different ecosystems is a result of genetic combinations along with complex metabolic reactions. For centuries, mankind has learned from nature and used its resources to improve the quality of our life. Therefore, the better understanding of the metabolites hole in living organisms' metabolism and/or in ecological interactions represent a potential strategy to streamline several processes under investigation, such as drug development. In this context, metabolomics has emerged as an indispensable tool to analyze the metabolites in biological systems through spectral information. Metabolomics has been applied to answer biological questions of increasing complexity, which demands robust methodologies to assure reproducibility and reliability in the results presented to the scientific community. Hence, this tutorial-review covers a step-by-step guide for metabolomics analysis, including free available tools. Data acquisition and the main steps of data processing are presented, and we also address commonly applied statistical analysis and data visualization approaches that can improve the comprehension and interpretation of complex datasets. In addition, we discuss some annotation tools and bring up good practices and how to apply them. This work provides background on resources and concepts needed from all the researchers interested in this topic. (AU) | |
| FAPESP's process: | 16/12304-2 - Chemical study of the symbiont fungus Leucoagaricus gongylophorus in co-culture with other fungus involved in the garden of leaf-cutter ants |
| Grantee: | Alana Kelyene Pereira |
| Support Opportunities: | Scholarships in Brazil - Doctorate (Direct) |
| FAPESP's process: | 22/01529-4 - Bioactivity of secondary metabolites produced by actinobacteria from the bulk soil of Paullinia cupana Mart. against soybean phytopathogens |
| Grantee: | Naydja Moralles Maimone |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 18/23144-1 - NMR-based metabolomics for monitoring metabolic alteration of citrus species caused by Phyllosticta citricarpa |
| Grantee: | Hocelayne Paulino Fernandes |
| Support Opportunities: | Scholarships abroad - Research Internship - Doctorate |
| FAPESP's process: | 12/25299-6 - Integrated studies for leaf cutting control |
| Grantee: | João Batista Fernandes |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 18/26066-1 - PHYTOCHEMICAL CHARACTERIZATION OF GERMPLASM SPECIES OF INSTITUTO AGRONÔMICO |
| Grantee: | Leila Gimenes |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 18/24865-4 - New approaches in metabolomics: 3D Molecular cartography of ascidians based on LC-MS/MS data |
| Grantee: | Anelize Bauermeister |
| Support Opportunities: | Scholarships abroad - Research Internship - Post-doctor |
| FAPESP's process: | 18/21936-8 - Characterization of the ecological function of the black yeast Phialophora fastigiata associated with leaf cutting ants |
| Grantee: | Alana Kelyene Pereira |
| Support Opportunities: | Scholarships abroad - Research Internship - Doctorate (Direct) |
| FAPESP's process: | 17/17648-4 - Integrative approach on the sustainable prospection of marine natural products: from diversity to anticancer compounds |
| Grantee: | Anelize Bauermeister |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 15/09208-9 - Study of variation of chemical profile of lemon (C. limon) of orange (C. sinensis) and Tahiti lime (C. latifolia) before and after inoculation with Phyllosticta citricicarpa and fungal strains related to infection by RNA viruses |
| Grantee: | Hocelayne Paulino Fernandes |
| Support Opportunities: | Scholarships in Brazil - Doctorate |