| Full text | |
| Author(s): |
de Godoy, Thais Almeida
;
de Lima, Eduardo Oliveira Venancio
;
Silveira, Giovanni Perez Machado
;
Rodrigues, Fabiola Souza
;
Sant'anna, Savio Stefani
;
Hatakeyama, Daniela Miki
;
Grego, Kathleen Fernandes
;
Tanaka-Azevedo, Anita Mitico
Total Authors: 8
|
| Document type: | Journal article |
| Source: | HELIYON; v. 10, n. 17, p. 12-pg., 2024-09-15. |
| Abstract | |
The genus Bothrops are considered Category 1 of medical importance by the World Health Organization, responsible for approximately 85 % of snakebites occurring throughout Brazil. Main factors determining snake venom variations can be genetics, diet, gender, geographic distribution, age, or even seasonality. In this study, we compared the composition of protein profile, biochemical activities, and immunorecognition of toxins present in the venom of eight adults of Bothrops species (B. alternatus, B. atrox, B. jararaca, B. jararacussu, B. leucurus, B. moojeni, B. neuwiedi and B. pauloensis). The following methods were used to analyze the venoms: protein dosage; electrophoresis in polyacrylamide gel containing SDS; High Performance Liquid Chromatography - Reverse Phase; enzymatic activities, western blotting and Enzyme Linked Immuno Sorbent Assay. The results show inter and intraspecific differences in the electrophoretic profile. LAAO and PLA(2) activities, in general, were higher in males than females and proteolytic activity was higher in females than males. The bothropic antivenom produced by Instituto Butantan recognized most of the protein bands in all Bothrops species analyzed, with only the regions between 37 and 25 kDa presenting lower intensity. A notable variability in the chromatograms was observed. Bothrops venom demonstrated inter-intraspecific disparities in protein composition and biochemical activity. (AU) | |
| FAPESP's process: | 22/15660-5 - Ontogenetic study of the venom of Bothrops alternatus snakes and analysis of phospholipase A2 inhibitors as a complementary therapy to serum therapy in bothropic accidents |
| Grantee: | Eduardo Oliveira Venancio de Lima |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 21/11707-4 - Evaluation of alternative in vitro methods to the use of animals for in vivo tests in the study of snake venoms: principle of the 3R's |
| Grantee: | Anita Mitico Tanaka-Azevedo |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 23/14327-3 - 3R'S AND VENOMICS: EVALUATION OF AN ALTERNATIVE IN VITRO METHOD TO THE USE OF ANIMALS IN THE STUDY OF SNAKE VENOMS |
| Grantee: | Anita Mitico Tanaka-Azevedo |
| Support Opportunities: | Regular Research Grants |