Living High-Training Low on Mice Bone Parameters Analyzed through Complex Network Approach

Author(s)

Beck, Wladimir Rafael ; Scariot, Pedro Paulo Menezes ; Papoti, Marcelo ; Pejon, Taciane Maria Melges ; Polisel, Emanuel Elias Camolese ; Manchado-Gobatto, Fulvia Barros ; Gobatto, Claudio Alexandre

• Document type: Journal article
• Source: INTERNATIONAL JOURNAL OF SPORTS MEDICINE; v. N/A, p. 9-pg., 2024-11-13.

Abstract

The aim of this study was to investigate the effect of 8 weeks of hypoxic exposition and physical training on healthy mice femur outcomes analyzed through conventional statistic and complex networks. The mice were divided into four groups, subjected to physical training (T; 40 min per day at 80% of critical velocity intensity) or not (N), exposed to hypoxic environment ("Living High-Training Low" model - LHTL; 18 h per day, FIO2 =19.5%; Hyp) or not (Nor). The complex network analysis performed interactions among parameters using values of critical "r" of 0.5 by Pearson correlations to edges construction, with Fruchterman-Reingold layout adopted for graph visualization. Pondered Degree, Betweenness, and Eigenvector metrics were chosen as centrality metrics. Two-way ANOVA, t-test and Pearson correlation were used with P<0.05. Femur phosphorus of T-Hyp was higher than all other groups (P<0.05) and correlated with bone density (r=0.65; P=0.042), bone mineral density (r=0.67; P=0.034) and% of mineral material (r=0.66, P=0.038). Overall, the complex network demonstrated improvements in bone volume, % of mineral material, bone density, and bone mineral density for T-Hyp over other groups. Association of physical training and hypoxia improved bone quality for healthy mice. (AU)

• FAPESP's process: 19/05115-7 - Effects of aerobic training and the hypoxic environment on molecular responses related to anaerobic and aerobic metabolism and acid-base properties in the hypothalamus and skeletal muscle of mice
• Grantee: Pedro Paulo Menezes Scariot
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 17/10201-4 - Effects of aerobic physical training and the availability of wide space housing on the spontaneous physical activity, central and peripheral metabolic and molecular responses
• Grantee: Claudio Alexandre Gobatto
• Support Opportunities: Regular Research Grants
• FAPESP's process: 15/00272-6 - Effects of caloric restriction mediated by SIRT3 in the aerobic and anaerobic capacities of mice: relations with the spontaneous activity, metabolic parameters and muscle mitochondrial function in aging process
• Grantee: Fúlvia de Barros Manchado Gobatto
• Support Opportunities: Scholarships abroad - Research
• FAPESP's process: 21/03951-2 - Melatonin as potentiator of the physical training effects on biomolecular parameters of energy metabolism under hypoestrogenism
• Grantee: Taciane Maria Melges Pejon
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 19/20930-9 - Effects of intermittent recovery hypoxia on molecular, metabolic and neuromuscular responses: implications for optimizing swimmer training
• Grantee: Marcelo Papoti
• Support Opportunities: Regular Research Grants
• FAPESP's process: 15/01362-9 - Effects of age, caloric restriction diet (Cr) and SIRT3 on spontaneous activity, behavior and oxidative mediators (NO, NOS and orexin) and hypothalamic mitochondrial respiration
• Grantee: Claudio Alexandre Gobatto
• Support Opportunities: Scholarships abroad - Research
• FAPESP's process: 19/08148-3 - Melatonin and physical performance: from ergogenic to accelerated skeletal muscle repair after damage induced by physical exercise
• Grantee: Wladimir Rafael Beck
• Support Opportunities: Regular Research Grants