Performance of clostridium species and autochthonous bacteria from citrus wastewater under different carbon sources to produce biofuels

Author(s)

da Silva, Daiana Camila ; Rodrigues, Caroline Varella ; Marin, Danieli Fernanda Canaver ; Lazaro, Carolina Zampol ; Jacobus, Ana Paula ; Pires, Lorena Oliveira ; Maintinguer, Sandra Imaculada

• Document type: Journal article
• Source: INTERNATIONAL JOURNAL OF HYDROGEN ENERGY; v. 49, p. 15-pg., 2023-12-08.

Abstract

This study evaluated the energy recovery of citrus wastes with pure cultures (Clostridium acetobutylicum ATCC 824, Clostridium beijerinckii ATCC 10132 -Inocula 1 and 2, respectively) and an autochthonous microbial (Inoculum 3) from citrus wastewater (CW) in anaerobic batch reactors to produce biofuels (H2 and ethanol) fed with different carbon sources (glucose, maltose, fructose, sucrose, xylose, lactose, starch, acetate, and glycerol) in addi-tion to CW. 4 conditions are tested: 1st -Inoculum 3, composed by Bacillus (20.44%), Clos-tridium (13.65%) and Lactobacillus (10.61%) was able to produce H2 with sucrose, glucose and fructose. 2nd -The highest metabolites were produced with TYA medium with Inocula 1 (374 mg L-1 of acetic acid) and 2 (611 mg L-1 of butanol). Inoculum 3 generated the highest ethanol (1571.8 mg L-1) in PYG medium. 3rd -In the same nutritional conditions (PYG), Inocula 1 and 2 produced H2 (36.80 and 64.10 mmol L-1, respectively) and acetic acid (246 and 201 mg L-1, respectively); the H2 was suppressed with inoculum 3 due to Lactobacillus genus. 4th H2 production was favored by autochthonous bacteria in CW. This study valued the autochthonous microorganisms in the possibility of bioconversion of organic matter into value-added products in addition to contribute to an environment-sustainable solu-tion for citrus processing. (c) 2023 Hydrogen Energy Publications LLC. Published by Elsevier Ltd. All rights reserved. (AU)

• FAPESP's process: 17/22401-8 - Fruit-refinery: obtention processes, characterization methods and generation of products derived from residues of the fruitculture
• Grantee: Nelson Ramos Stradiotto
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 17/16795-3 - Recovery of residues from biodiesel production to bioenergy generation in anaerobic systems integrated
• Grantee: Sandra Imaculada Maintinguer
• Support Opportunities: Regular Research Grants