| Full text | |
| Author(s): |
Orzari, Luiz Otavio
;
Brazaca, Lais Canniatti
;
Janegitz, Bruno Campos
Total Authors: 3
|
| Document type: | Journal article |
| Source: | Microchimica Acta; v. 191, n. 11, p. 13-pg., 2024-11-01. |
| Abstract | |
This work comprehends the development and characterization of a carbon black-based electrode modified with Au microflowers to increase its effect as a capacitance biosensor for the determination of PARK7/DJ-1. Due to its high surface-to-volume ratio and biocompatibility, Au particles are suitable for antibody binding, and by monitoring surface capacitance, it is possible to identify the immune-pair interaction. Au microflowers allowed the adequate immobilization of Parkinsonian-related proteins: PARK7/DJ-1 and its antibody. The protein is associated with several antioxidant mechanisms, but its abnormal concentrations or mutations can be the cause of the loss of dopaminergic neurons, leading to Parkinson's disease. The device was characterized by scanning electron microscopy and cyclic voltammetry, revealing the flower-like structures and the electrochemically-interest enhancements they provide, such as increased heterogeneous electron transfer rate coefficient and electroactive area. The self-assembled monolayers of different molecules were optimized with the aid of 22 central composite experiments and a linear calibration curve was obtained between 0.700 and 120 ng mL-1 of PARK7/DJ-1, with a limit of detection of 0.207 ng mL-1. The data confirms that the addition of Au microflowers enhanced the electrochemical signal of the device, as well as allowed for the determination of an early stage Parkinson's disease biomarker with appreciable analytical performance. (AU) | |
| FAPESP's process: | 19/23342-0 - Development of novel immunosensor architechtures for the detection of Huntington and Parkinson Diseases biomarkers |
| Grantee: | Luiz Otávio Orzari |
| Support Opportunities: | Scholarships in Brazil - Doctorate (Direct) |
| FAPESP's process: | 23/10141-2 - Novel wearable biosensors for non-invasive analysis of protein biomarkers |
| Grantee: | Laís Canniatti Brazaca |
| Support Opportunities: | Research Grants - Young Investigators Grants |
| FAPESP's process: | 23/06793-4 - Development of applied multiplex electrochemical systems for detection of biomarkers related to cardiovascular diseases |
| Grantee: | Bruno Campos Janegitz |
| Support Opportunities: | Regular Research Grants |