Flavonoid quantitative HPLC-DAD analysis of Petiveria alliacea

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Henrique, Camila Yamasita ; Pereira, Ina Blaine de Alvarenga ; Afonso, Laura Fernandes ; Barosela, Jose Ricardo ; Silva, Sarah Cristina Andrade ; Alves, Osvaine Junior Alvarenga ; Cunha, Wilson Roberto ; Silva, Marcio Luis Andrade e ; Januario, Ana Helena ; Leonel, Alexandre Henrique ; Nader, Talita Thomaz ; Groppo, Milton ; Pereira, Ana Maria Soares ; Pauletti, Patricia Mendonca

• Document type: Journal article
• Source: Journal of Liquid Chromatography & Related Technologies; v. 47, n. 16-20, p. 8-pg., 2024-06-27.

Abstract

Petiveria alliacea L. (Petiveriaceae), a medicinal plant found in tropical regions of South and Central America, the Caribbean, and Africa, is mainly used to treat inflammatory process and respiratory problems. In this study, we have evaluated the antibacterial activity of P. alliacea crude extract and fractions, investigated the chemical composition, and developed and validated a simple method to quantify flavanones. To verify the antibacterial activity, we determined the minimum inhibitory concentration (MIC) against Staphylococcus aureus and Escherichia coli. Phytochemical investigations were carried out by chromatographic and spectroscopic analysis. To quantify astilbin and engeletin, we developed a method based on HPLC coupled to a diode array detector (DAD) and validated it. The P. alliacea crude extracts and fractions exhibited MIC ranged from 10 to 25 mg/mL. Chemical investigation of P. alliacea revealed the presence of trans-N-methyl-4-methoxyproline (1), ethyl-beta-galactoside (2), ethyl-alpha-galactoside (3), and ethyl-beta-fructofuranoside (4). The developed HPLC-DAD method proved suitable for determining flavonoids in P. alliacea: it presented good linearity, specificity, and satisfactory accuracy and precision. The developed HPLC-DAD method can quantify the P. alliacea chemical markers. The isolated compounds (2-4) have been described in P. alliacea for the first time. (AU)

• FAPESP's process: 17/24860-0 - Acute toxicity evaluation of Stizophyllum perforatum leaves crude ethanol extract in vivo and chemical study associated with the evaluation of antiparasitic and cytotoxic activities in vitro
• Grantee: Osvaine Junior Alvarenga Alves
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 16/10313-4 - Phytochemistry study and evaluation of the schistosomicidal, cytotoxicity and leishmanicidal activities from cerrado plants
• Grantee: Patricia Mendonça Pauletti
• Support Opportunities: BIOTA-FAPESP Program - Regular Research Grants
• FAPESP's process: 16/06260-2 - Integrative systematic studies in Neotropical families, with emphasis on Rutaceae, Asteraceae and Rubiaceae
• Grantee: Milton Groppo Júnior
• Support Opportunities: Regular Research Grants
• FAPESP's process: 21/05175-0 - The phytochemical study, development, and validation of an analytical method by HPLC-DAD for analysis of the major constituents of Petiveria alliacea
• Grantee: Ina Blaine de Alvarenga Pereira
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 22/12597-0 - Diversification and enhancement of the collections on the hebaria in the State of São Paulo, with increase of their virtual availability and spatial analysis, for advances in documentation of the Neotropical biodiversity
• Grantee: José Rubens Pirani
• Support Opportunities: BIOTA-FAPESP Program - Thematic Grants