Gelatin Carbon Dots Interaction with Nitrosyl Ruthenium Complex: Fluorescence Quenching and Chemiluminescence Mechanisms

Author(s)

Astolfi, Bianca Soares ; Bessas, Naiara Cristina ; Graminha, Angelica Ellen ; Becceneri, Amanda Blanque ; da Silva, Roberto Santana ; de Lima, Renata Galvao

• Document type: Journal article
• Source: Journal of Fluorescence; v. 34, n. 6, p. 13-pg., 2023-11-10.

Abstract

Carbon dots (CDs) exhibit luminescence, biocompatibility, and higher water solubility. This material has been developed for biological applications, specifically in bioimaging. In this work, the gelatin carbon dots (CDg) was obtained from commercial gelatin using a hydrothermal method in domestic microwave, and the suppression fluorescent mechanism were enhanced by the addition of the [Ru-II(bdq)(NO)(tpy)](3+) (Rubdq-NO+) complex ion. After purification through a dialysis bag, the resulting CDs (CDg) exhibit fluorescent emission at 400 nm and maintained fluorescence stability in an aqueous solution (pH=7) for 30 days under 5 degrees C. Fluorescence quenching studies revealed an electrostatic interaction between the negative charge from CDg (delta=-20 mV) and the positively charged nitrosyl (NO+) ligand of the ruthenium complex (Rubdq-NO+), resulting in quenching of the CDg fluorescence due to the inner filter effects (IFE). The chemiluminescence reaction of CDg and Rubdq-NO-CDg in presence of norepinephrine (NOR) were evaluated. NOR in PBS are liable to undergo spontaneous oxidation to quinone form (NOR-quinone). CDg are believed interact with NOR-quinone and an electron transfer occur obtained CDg+ accompanied to green emission fluorescence (520 nm). While for Rubdq-NO-CDg in presence of NOR, the green emission occurs accompanied by NO0 release using DAF-2 probe. (AU)

• FAPESP's process: 20/03367-6 - Phototherapy using ruthenium-phthalocyanine complexes as generators of reactive oxygen and nitrogen species: study of the mechanism of action in vitro in different 2D and 3D models of cancer cells
• Grantee: Amanda Blanque Becceneri
• Support Opportunities: Scholarships in Brazil - Post-Doctoral