| Full text | |
| Author(s): |
Florezi, Giovanna P.
;
Labate, Monica Teresa Veneziano
;
Labate, Carlos Alberto
;
Barone, Felippe P.
;
Lourenco, Silvia Vanessa
Total Authors: 5
|
| Document type: | Journal article |
| Source: | ORAL DISEASES; v. N/A, p. 13-pg., 2025-03-27. |
| Abstract | |
Objectives: Primary Sj & ouml;gren's disease (SjD) is a slow-progressing autoimmune disease that affects salivary and lacrimal glands, causing dry eyes (xerophthalmia) and dry mouth (xerostomia). Diagnosing SjD involves clinical, serological, and histological assessments, but reliable biomarkers are lacking. Methods: This study analyzed the metabolic and proteomic profiles of 19 female SjD patients (based on ACR-EULAR criteria) compared to 20 healthy individuals. Saliva and blood samples were analyzed using mass spectrometry and chromatography. Proteomic analysis was performed with the nanoElute nanoflow system coupled to a timsTof-Pro mass spectrometer, and metabolic profiling with a GC-TOF/MS Pegasus HT. Results: Statistical tests identified significant differences in metabolites and proteins between SjD patients and controls. Metabolome analysis revealed changes in amino acid synthesis, purine and lipid metabolism, and exposure to external compounds. Proteomic analysis indicated immune-related proteins and inflammatory lipid metabolism. GNAI2, B2MG, NGAL, SLUR2, HS90, SODC, and A2GL emerged as potential biomarkers for SjD. Conclusion: This study demonstrates the potential of high-performance techniques in identifying biomarkers for SjD diagnosis and prognosis. Data are available via ProteomeXchange (PXD055629). (AU) | |
| FAPESP's process: | 17/11806-7 - Sialadenitis in Sjögren's Syndrome and lupus erythematosus: clinical-pathological correlation and proteomic and metabolomic analysis of saliva |
| Grantee: | Silvia Vanessa Lourenço |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 18/00419-5 - Functional genomics: reverse engineering using high-throughput methods for serological and salivary analysis in patients with Sjögren's syndrome |
| Grantee: | Giovanna Piacenza Florezi |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 22/12807-5 - Xerostomia in pre and post-menopause and its interface with women's oral health: investigation from bench to bedside |
| Grantee: | Silvia Vanessa Lourenço |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 19/12702-6 - Lupus sialadenitis: analysis of salivary metabolites and its possible relationship with peculiar aspects of salivary gland morphology |
| Grantee: | Felippe Pereira Barone |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |