The Development and Validation of a Novel HPLC-DAD Method for the Quantification of Icaridin in Insect Repellent Formulations

Author(s)

Farias, Fernanda Fernandes ; Barbara, Maria Cristina Santa ; Martins, Valeria Adriana Pereira ; Silva, Mariana Sbaraglini Garcia ; Silva, Vanessa Cristina Martins ; Andreo-Filho, Newton ; Lopes, Patricia Santos ; Leite-Silva, Vania Rodrigues

• Document type: Journal article
• Source: PROCESSES; v. 13, n. 3, p. 12-pg., 2025-02-22.

Abstract

The quality control of insect repellents contributes to the population's health since these products prevent mosquito bites and vector-borne diseases. In this study, we developed and validated a novel analytical method using high-performance liquid chromatography with a diode array detector (HPLC-DAD) for the quantification of icaridin in insect repellent lotions. The analysis was performed on a phenyl chromatographic column 150 x 4.6 mm, 3.5 mu m and stabilized at 30 degrees C. The detection of icaridin was achieved at 4.5 min with a 20 mu L injection volume of the samples. The active ingredient was extracted from the lotion samples with isopropanol and water (50:50 v/v) and then diluted to the working concentration at 0.6 mg/mL with the mobile phase. The calibration curve was linear in the concentration range of 0.1 to 1.2 mg/mL. The method was robust, specific and precise (relative standard deviations-RSD < 2%). The accuracy of the method was demonstrated by icaridin recovery. The limit of detection and quantification were 0.03 mg/mL and 0.1 mg/mL, respectively. The present report puts forward a novel analytical method for the quantification of icaridin, contributing to improving the quality control and efficacy of marketed formulations and their different presentations such as lotions, gels and sprays, demonstrating its good applicability. (AU)