| Full text | |
| Author(s): |
Miranda, Felipe de Souza
;
Azevedo Neto, Nilton Francelosi
;
Koga-Ito, Cristiane Yumi
;
Pessoa, Rodrigo Savio
Total Authors: 4
|
| Document type: | Journal article |
| Source: | PHYSICA SCRIPTA; v. 100, n. 7, p. 13-pg., 2025-07-01. |
| Abstract | |
Plasma-Activated Saline Solutions (PAS), derived from Plasma-Activated Water (PAW), are increasingly recognized for their versatile applications in infection control, wound healing, and sterilization. These solutions are produced by exposing liquids to cold plasma, generating a rich mixture of reactive oxygen and nitrogen species (RONS) and reactive chlorine species (RCS). This study investigates the production of PAS using a coaxial dielectric barrier discharge (DBD) operating with compressed air and evaluates the impact of varying sodium chloride (NaCl) concentrations (0.9%, 1.8%, 3.6%, and 7.2%) on the generation of RONS and RCS. Deionized water (DI water) was also activated as a baseline comparison. Physicochemical properties, including pH, oxidation-reduction potential (ORP), conductivity, and total dissolved solids (TDS), were measured before and after plasma activation. Real-time UV-Vis and Raman spectroscopy were employed to monitor the formation and stability of reactive species. To complement the UV-Vis data, additional analytical methods, such as colorimetric test strips and estimations based on pH and equilibrium constants, were employed. The analyses revealed that NaCl concentration significantly influences the composition and behavior of PAS, with lower concentrations favoring the generation of RONS such as nitrites ( NO2- ) and nitrates ( NO3- ), while higher concentrations modulate the production of RCS like hypochlorous acid (HClO). The results demonstrated a pronounced decrease in pH and an increase in ORP, indicating enhanced oxidative and antimicrobial potential. Spectroscopic analyses revealed distinct patterns of RONS formation, including NO3- , NO2- , H2O2, and HClO, with notable interactions between RCS and RONS at higher NaCl concentrations. These findings underscore the dynamic interplay between ionic strength, reactive species production, and solution stability, providing critical insights into optimizing PAS for biomedical applications. The study highlights PAS's potential as eco-friendly, residue-free alternatives for disinfection and wound care, offering tailored properties based on NaCl concentration to meet specific therapeutic needs. (AU) | |
| FAPESP's process: | 21/14181-3 - Development and study of a plasma system generated by dielectric barrier discharge (DBD) for continuous activation of liquids applied in dental treatments |
| Grantee: | Felipe de Souza Miranda |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 22/11544-0 - Technological EMU: acquisition of a near-field scanning optical microscopy system coupled with atomic force microscopy and tip-enhanced Raman spectroscopy for chemical and physical imaging at micro and nanoscale |
| Grantee: | Rodrigo Savio Pessoa |
| Support Opportunities: | Research Infrastructure Program - Technology and Innovation |
| FAPESP's process: | 19/05856-7 - Use of low temperature atmospheric pressure plasma in dentistry: from laboratory bench to clinics |
| Grantee: | Cristiane Yumi Koga Ito |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 23/02268-2 - Generation and characterization of different liquids activated with plasma for use in endodontic treatment |
| Grantee: | Nilton Francelosi Azevedo Neto |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |