| Full text | |
| Author(s): |
da Silva, Daniel Jose
;
Mendes Cobe, Gabriella
;
Colonese Vlasman, Raphael
;
Catalani, Luiz Henrique
Total Authors: 4
|
| Document type: | Journal article |
| Source: | ACS POLYMERS AU; v. N/A, p. 12-pg., 2025-10-22. |
| Abstract | |
Polypeptides are attractive, renewable, and biocompatible materials for broad potential biomedical, pharmaceutical, and regenerative medicine applications. In this study, star-shaped 3-arm amphiphilic polypeptides with self-assembly characteristics were synthesized by ring-opening polymerization (ROP) of N-carboxyanhydride (NCA), incorporating L-leucine and L-lysine to form a core-shell structure. These polymers were thoroughly characterized through various techniques, revealing that their rheological behavior and self-assembly are influenced by factors such as the length of the star-shaped arms, the proportion of hydrophobic amino acids, and the surrounding pH. The synthesized polypeptides can self-assemble into five distinct secondary structures, mimicking natural proteins. Our findings evidence that the design of the block size of the hydrophilic L-lysine core and hydrophobic L-leucine shell shapes the ability to form a physical hydrogel, exhibiting shear-thinning rheological characteristics and a rapid mechanical response. The internal microstructure of the hydrogel is based on a supramolecular self-assembly structure consisting of highly connected nanofibrils. (AU) | |
| FAPESP's process: | 20/15986-2 - Synthesis and characterization of photocrosslinkable and functionalizable polypeptides for 3D print |
| Grantee: | Raphael Colonese Vlasman |
| Support Opportunities: | Scholarships in Brazil - Doctorate (Direct) |
| FAPESP's process: | 18/13492-2 - Synthetic and natural scaffolds applied to regenerative medicine |
| Grantee: | Luiz Henrique Catalani |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 22/16427-2 - Photo-crosslinkable N-carboxyanhydride derived polypeptides with pseudoplastic characteristics for extrusion-based 3D printing |
| Grantee: | Daniel José da Silva |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |