Structural investigations on model systems based on amphiphilic peptides

• Grant number: 13/12674-6
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Effective date (Start): December 01, 2013
• Effective date (End): June 09, 2016
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Physical-Chemistry
• Principal Investigator: Wendel Andrade Alves
• Grantee: Emerson Rodrigo da Silva
• Host Institution: Centro de Ciências Naturais e Humanas (CCNH). Universidade Federal do ABC (UFABC). Ministério da Educação (Brasil). Santo André , SP, Brazil
• Associated scholarship(s): 14/03514-8 - Structural investigations on model-systems based on amphiphilic peptides, BE.EP.PD

Abstract

We propose the synthesis and characterization of new peptide nanostructures obtained from self-assembly reactions. Inedited peptide sequences of bola-amphiphile and lipopeptide type will be prepared. The choice of these classes relies on the rich polymorphism of mesophases they are able to form and on their ability to establish biointerfaces through molecular recognition, increasing their range of applications significantly. We intend to evaluate the formation of these systems and contribute to elucidate the interactions appearing in their construction. Small- and wide-angle scattering techniques (SAXS and WAXS) will be used to probe structural aspects in order to provide insights on the physicochemical mechanisms implied in the self-assembly. After determining the optimal conditions for obtaining the arrays, their respective phase diagrams will be raised. To achieve this, techniques providing complementary information in both direct and reciprocal space will be used. Besides data on structure and morphology, these techniques will supply parameters on elastic and thermodynamic properties. This information, combined with data from in silico experiments performed by collaborators, will contribute for elucidating basic mechanisms involved in the building of the nanostructures. In following, structural changes will be evaluated in the peptides by varying the amino acid residues. The goal is to obtain different supramolecular organizations with potential applications in detection architectures. Furthermore, this hierarchical assembly could be followed in situ by using SAXS techniques, as previously demonstrated.

Scientific publications

(10)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SOUZA, SIVONEY F.; KOGIKOSKI, JR., SERGIO; SILVA, EMERSON R.; ALVES, WENDEL A.. Nanostructured Antigen-Responsive Hydrogels Based on Peptides for Leishmaniasis Detection. Journal of the Brazilian Chemical Society, v. 28, n. 9, p. 1619-1629, SEP 2017. (08/57805-2, 12/01933-8, 13/12674-6, 15/24018-1)

BIANCHI, ROBERTA C.; DA SILVA, EMERSON RODRIGO; DALL'ANTONIA, LUIZ H.; FERREIRA, FABIO FURLAN; ALVES, WENDEL ANDRADE. A Nonenzymatic Biosensor Based on Gold Electrodes Modified with Peptide Self-Assemblies for Detecting Ammonia and Urea Oxidation. Langmuir, v. 30, n. 38, p. 11464-11473, SEP 30 2014. (08/10537-3, 13/12997-0, 13/12674-6, 11/05374-0, 08/57805-2)

SOUZA, SIVONEY F.; KOGIKOSKI, SERGIO, JR.; SILVA, EMERSON R.; ALVES, WENDEL A.. Nanostructured Antigen-Responsive Hydrogels Based on Peptides for Leishmaniasis Detection. Journal of the Brazilian Chemical Society, v. 28, n. 9, p. 11-pg., 2017-09-01. (13/12674-6, 08/57805-2, 15/24018-1, 12/01933-8)

SOUZA, MARCIA I.; SILVA, EMERSON R.; JAQUES, YGOR M.; FERREIRA, FABIO F.; FILETI, EUDES E.; ALVES, WENDEL A.. The role of water and structure on the generation of reactive oxygen species in peptide/hypericin complexes. JOURNAL OF PEPTIDE SCIENCE, v. 20, n. 7, p. 9-pg., 2014-07-01. (08/10537-3, 13/12997-0, 08/57805-2, 13/17193-6, 13/12674-6)

SOUZA, MARCIA I.; SILVA, EMERSON R.; JAQUES, YGOR M.; FERREIRA, FABIO F.; FILETI, EUDES E.; ALVES, WENDEL A.. The role of water and structure on the generation of reactive oxygen species in peptide/hypericin complexes. JOURNAL OF PEPTIDE SCIENCE, v. 20, n. 7, SI, p. 554-562, JUL 2014. (08/10537-3, 13/17193-6, 13/12997-0, 13/12674-6, 08/57805-2)

KOGIKOSKI, JR., SERGIO; LIBERATO, MICHELLE S.; FACTORI, IRINA M.; DA SILVA, EMERSON R.; OLIVEIRA, CRISTIANO L. P.; ANDO, ROMULO A.; ALVES, WENDEL A.. Polycaprolactone-Polyaniline Blend: Effects of the Addition of Cysteine on the Structural and Molecular Properties. Journal of Physical Chemistry C, v. 121, n. 1, p. 863-877, JAN 12 2017. (08/57805-2, 13/12997-0, 12/01933-8, 12/15481-1, 15/24018-1, 13/12674-6)

HAMLEY, IAN W.; BURHOLT, SAMUEL; HUTCHINSON, JESSICA; CASTELLETTO, VALERIA; DA SILVA, EMERSON RODRIGO; ALVES, WENDEL; GUTFREUND, PHILIPP; PORCAR, LIONEL; DATTANI, RAJEEV; HERMIDA-MERINO, DANIEL; et al. Shear Alignment of Bola-Amphiphilic Arginine-Coated Peptide Nanotubes. Biomacromolecules, v. 18, n. 1, p. 141-149, JAN 2017. (13/12674-6, 15/24018-1)

SOARES, B. M.; AGUILAR, A. M.; SILVA, E. R.; COUTINHO-NETO, M. D.; HAMLEY, I. W.; REZA, M.; RUOKOLAINEN, J.; ALVES, W. A.. Chiral organocatalysts based on lipopeptide micelles for aldol reactions in water. Physical Chemistry Chemical Physics, v. 19, n. 2, p. 1181-1189, 2017. (15/24018-1, 08/57805-2, 13/12674-6)

SILVA, EMERSON R.; COONEY, GARY; HAMLEY, IAN W.; ALVES, WENDEL A.; LEE, SHANNON; O'CONNOR, BRENDAN F.; REZA, MEHEDI; RUOKOLAINEN, JANNE; WALLS, DERMOT. Structural behaviour and gene delivery in complexes formed between DNA and arginine-containing peptide amphiphiles. SOFT MATTER, v. 12, n. 45, p. 9158-9169, 2016. (15/24018-1, 13/12674-6, 14/03514-8)

LIBERATO, M. S.; KOGIKOSKI, JR., S.; DA SILVA, E. R.; DE ARAUJO, D. R.; GUHA, S.; ALVES, W. A.. Polycaprolactone fibers with self-assembled peptide micro/nanotubes: a practical route towards enhanced mechanical strength and drug delivery applications. JOURNAL OF MATERIALS CHEMISTRY B, v. 4, n. 8, p. 1405-1413, 2016. (12/15481-1, 13/12997-0, 13/12674-6, 12/01933-8, 08/57805-2)