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Uncovering chemical novelty from freshwater filamentous Cyanobacteria

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Author(s):
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Weiss, Marcio B. ; Yoon, Jaewon ; Domingues, Joao P. B. ; Jacinavicius, Fernanda R. ; Freirec, Vitor F. ; V. Domingosd, Helori ; de Moraes Junior, Manoel O. ; Gonsalves, Camila M. C. ; Castro, Sandra R. ; Lindoso, Jose A. L. ; Maria-Engler, Silvya S. ; V. Costa-Lotufo, Leticia ; Borgesg, Ricardo M. ; Berlinck, Roberto G. S. ; Crnkovic, Camila M.
Total Authors: 15
Document type: Journal article
Source: Fitoterapia; v. 189, p. 8-pg., 2026-03-01.
Abstract

Natural products are a valuable source of molecules with therapeutic and biotechnological applications. However, the complexity of biological matrices and the increasing rates of rediscovery pose significant challenges in the search for new bioactive compounds. Exploring novel specimens from biodiversity and utilizing modern cheminformatics tools are essential to advancing natural product discovery. In this study, we employed metabolomics and bioassays to investigate the potential of filamentous cyanobacteria collected in Brazil as a source of new bioactive secondary metabolites. Extracts and fractions from eight freshwater cyanobacterial strains were tested for their general toxicity against Artemia salina, as well as antiparasitic and cytotoxic activities and analyzed by UHPLC-HRMS-MS/MS. Molecular networks were constructed from MS/MS data using the GNPS platform, and dereplication was guided by DAFdiscovery results for feature prioritization. Annotation and dereplication were conducted using SIRIUS combined with manual analysis of HRMS and MS/MS spectra. Our study identified three cyanobacterial strains as producers of potentially novel and bioactive metabolites for in-depth investigations. (AU)

FAPESP's process: 20/15042-4 - Antiproliferative and cell invasion inhibitor potencial of new copper (II) complexes in human Melanoma with resistance to BRAF inhibitors phenotype
Grantee:Manoel Oliveira de Moraes Junior
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 24/02448-3 - Analysis of the impact of cyanobacteria inoculation on the maize rhizosphere community
Grantee:Fernanda Rios Jacinavicius
Support Opportunities: Scholarships in Brazil - Technical Training Program - Technical Training
FAPESP's process: 22/02872-4 - Integrated strategies for natural product discovery from Brazilian cyanobacteria
Grantee:Camila Manoel Crnkovic
Support Opportunities: Research Grants - Initial Project
FAPESP's process: 20/07710-7 - Biotechnological prospection of natural products from cyanobacteria
Grantee:Camila Manoel Crnkovic
Support Opportunities: BIOTA-FAPESP Program - Regular Research Grants
FAPESP's process: 19/17721-9 - The role of Chemistry in holobiont adaptation
Grantee:Roberto Gomes de Souza Berlinck
Support Opportunities: Research Projects - Thematic Grants