| Full text | |
| Author(s): |
dos Santos Junior, Adeilso Bispo
;
Tamayose, Cinthia I.
;
Ferreira, Marcelo J. P.
;
Belchor, Mariana N.
;
Costa, Caroline R. C.
;
de Oliveira, Marcos Antonio
;
Toyama, Marcos Hikari
Total Authors: 7
|
| Document type: | Journal article |
| Source: | Molecules; v. 25, n. 2, p. 18-pg., 2020-01-02. |
| Abstract | |
Bioaffinity capturing of molecules allows the discovery of bioactive compounds and decreases the need for various stages in the natural compound isolation process. Despite the high selectivity of this technique, the screening and identification methodology depends on the presence of a protein to capture potential ligands. However, some proteins, such as snake secretory phospholipase A2 (sPLA2), have never been investigated using this approach. The purpose of this study was to evaluate the use of a new method for screening natural compounds using a bioaffinity-guided ultrafiltration method on Crotalus durissus terrificus sPLA2 followed by HPLC-MS to identify the compounds, and this method could be used to discover new anti-inflammatory compounds from the various organisms originating from biodiversity. Different extracts were selected to evaluate their ability to inhibit sPLA2 activity. The extracts were incubated with sPLA2 and the resulting mixture was ultrafiltrated to elute unbound components. The resulting compounds were identified by HPLC-MS. We identified hispidulin as one of the components present in the Moquiniastrum floribundum leaf and evaluated the ability of this isolated compound to neutralize the inflammatory activity of sPLA2 from Crotalus durissus terrificus. (AU) | |
| FAPESP's process: | 14/21593-2 - Baccharis L. (Asteraceae) and endophytic fungi: secondary metabolites and biological activities |
| Grantee: | Marcelo José Pena Ferreira |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 17/20291-0 - Characterization of the proinflammatory activity of a new serine protease (cdtsp-2) purified from the total venom of Crotalus durissus terrificus |
| Grantee: | Marcos Hikari Toyama |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 14/20932-8 - PHYTOCHEMISTRY, CHEMOSYSTEMATIC STUDY AND BIOLOGICAL ACTIVITIES OF Moquiniastrum AND Richterago SPECIES |
| Grantee: | Cinthia Indy Tamayose |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 17/19942-7 - Search for inhibitors of the peroxirredoxin system from pathogens and humans |
| Grantee: | Marcos Antonio de Oliveira |
| Support Opportunities: | Regular Research Grants |