| Full text | |
| Author(s): |
Fernandes Patta, Ana C. M.
;
Mathews, Patrick D.
;
Madrid, Rafael R. M.
;
Rigoni, Vera L. S.
;
Silva, Emerson R.
;
Mertins, Omar
Total Authors: 6
|
| Document type: | Journal article |
| Source: | International Journal of Biological Macromolecules; v. 148, p. 15-pg., 2020-04-01. |
| Abstract | |
The production of efficient micro and nanoparticles of pH-responsive and mucoadhesive properties is of high scrutiny. We produced a drug carrier bioparticle providing such structural features. Thereby, protonated chitosan bearing chemically bonded arginine was interacted with ionized alginate, leading to the assembling of colloidal particles of specific characteristics. Ideally, the ratio of chitosan-arginine monomers to alginate monomer is 1.6 when the biopolymers are highly charged, providing electrostatic interaction with Gibbs energy compensation around similar to 14 kcal/mol. Both size and surface charge of the bioparticle respond to pH variation, leading to structures of nano to micro hydrodynamic diameters and of positive, nearly neutral and negative zeta potential, with nanoscopic structure changing from mass to surface fractals. The inclusion of two hydrophobic model drugs provided some specific physicochemical features. Following freeze-drying, the bioparticles present both irregular shape and surface morphology, but an overall similar dry structure. An in vivo study of oral administration to teleost fish revealed that the bioparticles attain the intestine mucus and further, the interaction with the intestinal mucosa is timely dependent thanks to the mucoadhesive property. The in vivo study endorsed that the bioparticle provides high compliance to freshwater ornamental fish, highlighting it as a material of promising application. (C) 2020 Elsevier B.V. All rights reserved. (AU) | |
| FAPESP's process: | 17/16722-6 - pH responsive nanoparticles for optimization of oral delivery of antiparasite drugs in the treatment of ornamental fish |
| Grantee: | Ana Carolina Monge Fernandes Patta |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 18/20482-3 - Diversity of microscopic cnidarians from the Amazon Basin with emphasis on phylogeny, systematic and interaction with their hosts |
| Grantee: | Patrick Mathews Delgado |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 16/24409-3 - Cell-Penetrating Peptides for Transport of Plasmid DNA and microRNA: from Nanoscopic Structure to Gene Delivery. |
| Grantee: | Emerson Rodrigo da Silva |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 15/23948-5 - Improvement of the polysaccharide chitosan properties for its application in liposomes and giant vesicles |
| Grantee: | Omar Mertins |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 16/13368-4 - Nanostructured systems: from membrane biomimetic models to carriers of bioactives |
| Grantee: | Karin Do Amaral Riske |
| Support Opportunities: | Research Projects - Thematic Grants |