| Full text | |
| Author(s): |
Santos-Garcia, Melissa de Oliveira
[1]
;
Simeao Resende, Rosangela Maria
[2]
;
Chiari, Lucimara
[2]
;
Zucchi, Maria Imaculada
[3]
;
de Souza, Anete Pereira
[1, 4]
Total Authors: 5
|
| Affiliation: | [1] Univ Estadual Campinas, Ctr Biol Mol & Engn Genet, BR-13083970 Campinas, SP - Brazil
[2] Empresa Brasileira Pesquisa Agropecuaria Embrapa, BR-79002970 Campo Grande, MS - Brazil
[3] Polo Apta Ctr Sul, BR-13400970 Piracicaba, SP - Brazil
[4] Univ Estadual Campinas, Dept Biol Vegetal, Inst Biol, BR-13083970 Campinas, SP - Brazil
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | EUPHYTICA; v. 178, n. 2, p. 185-193, MAR 2011. |
| Web of Science Citations: | 9 |
| Abstract | |
Stylosanthes capitata and S. guianensis are important forage legumes for tropical areas. The only available estimates of S. capitata and S. guianensis outcrossing rates were based on morphological markers, and the genus is considered as being mainly self-pollinated. Here we describe an estimation of the outcrossing rate in S. capitata and S. guianensis using microsatellite markers. The outcrossing rates were estimated in S. capitata and S. guianensis open-pollinated populations of 20 progenies consisting of ten individuals each. The multi locus outcrossing rate for S. capitata was estimated using 10 polymorphic loci, whereas five microsatellites were used for S. guianensis. The multi locus outcrossing rates for S. capitata and S. guianensis were 31 and 26%, respectively, suggesting a mixed mating system with predominance of autogamy. Comparison of single locus and multi locus estimates of outcrossing rates indicated that little inbreeding other than selfing occurred. The estimated Wright's fixation index of the parental generation was lower than expected based on the multi locus outcrossing rate, possibly resulting from the use of some heterozygous breeding genotypes for the study. The data on the outcrossing rate described here are potentially useful for breeding programs and for maintenance of germplasm collections of these Stylosanthes species. (AU) | |