| Full text | |
| Author(s): |
de Oliveira, Patricia Rosa
[1]
;
Bechara, Gervasio Henrique
[2]
;
Denardi, Sandra Eloisi
[1]
;
Pizano, Marcos Aparecido
[3]
;
Camargo Mathias, Maria Izabel
[1]
Total Authors: 5
|
| Affiliation: | [1] Sao Paulo State Univ UNESP, Inst Biosci, Dept Biol, BR-13506900 Rio Claro, SP - Brazil
[2] Sao Paulo State Univ UNESP, Dept Anim Pathol, Fac Agron & Vet Sci, BR-14884900 Jaboticabal, SP - Brazil
[3] Sao Paulo State Univ UNESP, Inst Biosci, Dept Ecol, BR-13506900 Rio Claro, SP - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | Experimental Parasitology; v. 127, n. 2, p. 418-422, FEB 2011. |
| Web of Science Citations: | 10 |
| Abstract | |
Chemical acaricides, especially fipronil (active ingredient of Frontline (R)), are still the most effective method to control tick populations. In this study, the effectiveness of fipronil was assessed in semi-engorged females of the tick Rhipicephalus sanguineus. A protocol for an in vitro bioassay (AIT) was developed, and the LC(50) (lethal concentration 50%) and 95% confidence interval were determined. Ticks were immersed in Petri dishes with different concentrations of fipronil or distilled water for 2 min, dried, and placed in an incubator for 7 days. Dead R. sanguineus females treated with the 14 concentrations of fipronil were counted daily. Mortality results were compared with the Probit analysis, and the LC(50) and 95% confidence interval were calculated, g (95): LC(50) = 9.647 (4.711 to 13.470). This study was aimed at developing a more appropriate and updated protocol for an in vitro bioassay (AIT - adult immersion test), and providing information on the toxic potential of fipronil (elimination of ectoparasites with lower concentrations) and sensitivity of ticks, especially R. sanguineus, a pest of great interest, due to its occurrence in urban environments. (C) 2010 Elsevier Inc. All rights reserved. (AU) | |