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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

The non-coding RNA BC1 is down-regulated in the hippocampus of Wistar Audiogenic Rat (WAR) strain after audiogenic kindling

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Author(s):
Goes Gitai, Daniel Leite [1] ; Fachin, Ana Lucia [2] ; Mello, Stephano Spano [2] ; Elias, Carol Fuzachi [3] ; Bittencourt, Jackson Cioni [3] ; Leite, Joao Pereira [4] ; da Silva Passos, Geraldo Aleixo [2] ; Garcia-Cairasco, Norberto [5] ; Paco-Larson, Maria Luisa [1]
Total Authors: 9
Affiliation:
[1] Univ Sao Paulo, Ribeirao Preto Sch Med, Dept Cellular & Mol Biol, BR-05508 Sao Paulo - Brazil
[2] Univ Sao Paulo, Ribeirao Preto Sch Med, Dept Genet, Mol Immunogenet Grp, BR-05508 Sao Paulo - Brazil
[3] Univ Sao Paulo, Inst Biomed Sci, Dept Anat, BR-05508 Sao Paulo - Brazil
[4] Univ Sao Paulo, Dept Neurosci & Behav, BR-05508 Sao Paulo - Brazil
[5] Univ Sao Paulo, Ribeirao Preto Sch Med, Dept Physiol, BR-05508 Sao Paulo - Brazil
Total Affiliations: 5
Document type: Journal article
Source: Brain Research; v. 1367, p. 114-121, JAN 7 2011.
Web of Science Citations: 11
Abstract

The aim of this study was to identify molecular pathways involved in audiogenic seizures in the epilepsy-prone Wistar Audiogenic Rat (WAR). For this, we used a suppression-subtractive hybridization (SSH) library from the hippocampus of WARs coupled to microarray comparative gene expression analysis, followed by Northern blot validation of individual genes. We discovered that the levels of the non-protein coding (npc) RNA BC1 were significantly reduced in the hippocampus of WARs submitted to repeated audiogenic seizures (audiogenic kindling) when compared to Wistar resistant rats and to both naive WARs and Wistars. By quantitative in situ hybridization, we verified lower levels of BC1 RNA in the GD-hilus and significant signal ratio reduction in the stratum radiatum and stratum pyramidale of hippocampal CA3 subfield of audiogenic kindled animals. Functional results recently obtained in a BC1-/- mouse model and our current data are supportive of a potential disruption in signaling pathways, upstream of BC1, associated with the seizure susceptibility of WARs. (C) 2010 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 05/56447-7 - Research through images from high field magnetic resonance aimed at studies in humans
Grantee:João Pereira Leite
Support Opportunities: Inter-institutional Cooperation in Support of Brain Research (CINAPCE) - Thematic Grants