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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Detection of DNA nucleotides on pretreated boron doped diamond electrodes

Full text
Author(s):
Garbellini, Gustavo S. [1] ; Uliana, Carolina V. [1] ; Yamanaka, Hideko [1]
Total Authors: 3
Affiliation:
[1] Sao Paulo State Univ UNESP, Inst Chem, BR-14800900 Araraquara, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Journal of the Brazilian Chemical Society; v. 22, n. 7, p. 1241-1245, 2011.
Web of Science Citations: 14
Abstract

The individual detection and equimolar mixture of DNA nucleotides guanosine monophosphate (GMP), adenosine monophosphate (AMP), thymidine (TMP) and cytidine (CMP) 5'-monophosphate using square wave voltammetry was performed on boron doped diamond (BDD) electrodes cathodically (Red-DDB) and anodically (Oxi-DDB) pretreated. The oxidation of individual DNA nucleotides was more sensitive on Oxi-BDD electrode. In a simultaneous detection of nucleotides, the responses of GMP, AMP, TMP and CMP were very adequate on both treated electrodes. Particularly, more sensitive and separate peaks for TMP and CMP on Oxi-BDD and Red-BDD electrodes, respectively, were observed after deconvolution procedure. The detection of nucleotides in aqueous solutions will certainly contribute for genotoxic evaluation of substances and hybridization reactions by immobilizing ss or ds-DNA on BDD surface. (AU)

FAPESP's process: 08/08990-1 - Studies of DNA damage by textile dyes and the protective capacity of flavonoids using electrochemical biosensor
Grantee:Carolina Venturini Uliana
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 09/08161-8 - Evaluation of organophosphate pesticides and DNA interactions using electrochemical biosensor
Grantee:Gustavo Stoppa Garbellini
Support Opportunities: Scholarships in Brazil - Post-Doctoral