Advanced search
Start date
Betweenand
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Expression of Circadian Clock and Melatonin Receptors within Cultured Rat Cardiomyocytes

Full text
Author(s):
Peliciari-Garcia, Rodrigo A. [1] ; Zanquetta, Melissa M. [2] ; Andrade-Silva, Jessica [1] ; Gomes, Dayane A. [3] ; Barreto-Chaves, Maria Luiza [3] ; Cipolla-Neto, Jose [1]
Total Authors: 6
Affiliation:
[1] Univ Sao Paulo, Dept Physiol & Biophys, Inst Biomed Sci, BR-05508900 Sao Paulo - Brazil
[2] Univ Sao Paulo, Lab Cellular & Mol Endocrinol, Sch Med, BR-05508900 Sao Paulo - Brazil
[3] Univ Sao Paulo, Dept Anat, Inst Biomed Sci, BR-05508900 Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: CHRONOBIOLOGY INTERNATIONAL; v. 28, n. 1, p. 21-30, 2011.
Web of Science Citations: 19
Abstract

Melatonin, the pineal gland hormone, provides entrainment of many circadian rhythms to the ambient light/dark cycle. Recently, cardiovascular studies have demostrated melatonin interactions with many physiological processes and diseases, such as hypertension and cardiopathologies. Although membrane melatonin receptors (MT1, MT2) and the transcriptional factor ROR alpha have been reported to be expressed in the heart, there is no evidence of the cell-type expressing receptors as well as the possible role of melatonin on the expression of the circadian clock of cardiomyocytes, which play an important role in cardiac metabolism and function. Therefore, the aim of this study was to evaluate the mRNA and protein expressions of MT1, MT2, and ROR alpha and to determine whether melatonin directly influences expression of circadian clocks within cultured rat cardiomyocytes. Adult rat cardiomyocyte cultures were created, and the cells were stimulated with 1 nM melatonin or vehicle. Gene expressions were assayed by real-time polymerase chain reaction (PCR). The mRNA and protein expressions of membrane melatonin receptors and RORa were established within adult rat cardiomyocytes. Two hours of melatonin stimulation did not alter the expression pattern of the analyzed genes. However, given at the proper time, melatonin kept Rev-erb alpha expression chronically high, specifically 12 h after melatonin treatment, avoiding the rhythmic decline of Rev-erb alpha mRNA. The blockage of MT1 and MT2 by luzindole did not alter the observed melatonin-induced expression of Rev-erb alpha mRNA, suggesting the nonparticipation of MT1 and MT2 on the melatonin effect within cardiomyocytes. It is possible to speculate that melatonin, in adult rat cardiomyocytes, may play an important role in the light signal transduction to peripheral organs, such as the heart, modulating its intrinsic rhythmicity. (Author correspondence: cipolla@icb.usp.br) (AU)

FAPESP's process: 09/52920-0 - Melatonin and the control of energy metabolism: central and peripheral actions and the circadian timing of the metabolic function
Grantee:José Cipolla Neto
Support type: Research Projects - Thematic Grants